추천 제품
Grade
for molecular biology
Quality Level
설명
TB; quick dissolve, dust-free formulation
무균
non-sterile
형태
powder
기술
microbiological culture: suitable
응용 분야
agriculture
food and beverages
microbiology
저장 온도
room temp
적합성
nonselective for Escherichia coli
nonselective for coliforms
관련 카테고리
일반 설명
Terrific Broth is a highly-referenced microbial growth medium used for the cultivation of E. coli. This nutrient-rich microbial broth contains glycerol, peptides, amino acids, water-soluble vitamins, and carbohydrates.
애플리케이션
Suitable for non-selective cultivation of E. coli strains for cloning, high yield DNA plasmid production and high yield production of recombinant proteins. Also suitable for selective cultivation when appropriate antibiotics are added.
Terrific broth has been used:
Terrific broth has been used:
- as a medium for selective growth of E.coli BL21 (DE3) V2RpRARE expressing MLL SET (nuclear proto-oncogene) domain in pET28GST-LIC
- to grow BL21 (DE3) cells containing plasmid expressing IL-8 (interleukin-8)
- Terrific broth is also suitable for use in bacterial suspension (Bacillus subtilis strain 1085B)
특징 및 장점
Terrific Broth EZMix powder provides:
- Granulated, dust-free format for safer handling and faster mixing
- A budget-friendly alternative to liquid
- Modified formulation (without glycerol)
- Easy scale-up using larger package sizes
성분
*Note some of the product sizes available are given in liquid measure. This is a powder format product and those sizes reflect the final reconstituted volume.
23.6g/L Yeast Extract
11.8g/L Tryptone
9.4g/L K2HPO4
2.2g/L KH2PO4
23.6g/L Yeast Extract
11.8g/L Tryptone
9.4g/L K2HPO4
2.2g/L KH2PO4
재구성
Suspend 24.1 g of powder and 4 mL of glycerol in sufficient deionized water to make 500 mL. Autoclave for 15 minutes at 121 °C to sterilize. Allow to cool before making additions, such as antibiotics (if desired).
법적 정보
관련 제품
제품 번호
설명
가격
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
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프로토콜
General protocols for growth of competent cells in microbial medium.
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