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Merck
모든 사진(1)

문서

T7791

Sigma-Aldrich

Thymidine 5′-triphosphate sodium salt solution

10 mM (pH 7.0)

동의어(들):

TTP

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모든 사진(1)

About This Item

CAS Number:
18423-43-3
MDL number:
MFCD00080338
UNSPSC 코드:
41106305
PubChem Substance ID:
24900477
NACRES:
NA.52

분석

≥99%

Quality Level

200

형태

liquid

농도

10 mM (pH 7.0)

색상

colorless

외래 활성

DNase, RNase, none detected

배송 상태

dry ice

저장 온도

−20°C

SMILES string

[Na].CC1=CN(C2CC(O)C(COP(O)(=O)OP(O)(=O)OP(O)(O)=O)O2)C(=O)NC1=O

InChI

1S/C10H17N2O14P3.Na.H/c1-5-3-12(10(15)11-9(5)14)8-2-6(13)7(24-8)4-23-28(19,20)26-29(21,22)25-27(16,17)18;;/h3,6-8,13H,2,4H2,1H3,(H,19,20)(H,21,22)(H,11,14,15)(H2,16,17,18);;

InChI key

KAELIXTVSANDSE-UHFFFAOYSA-N

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일반 설명

Thymidine 5′-triphosphate sodium salt solution is used in DNA synthesis reactions such as PCR, DNA sequencing, cDNA synthesis and molecular cloning techniques.

애플리케이션

Thymidine 5′-triphosphate sodium salt solution has been used:

  • as a standard to quantify each dNTP from cellular lysates by the liquid chromatography-mass spectrometry (LC-MS/MS) method
  • as a component of the dNTP mix for labeling of ribosomal 18s rDNA probe and telomeric (TTAGG)n probe
  • for routine PCR amplifications

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

개인 보호 장비

Eyeshields, Gloves, type N95 (US)

시험 성적서(COA)

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Sigma-Aldrich

D7295

Deoxynucleotide Mix, 10 mM

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Sigma-Aldrich

DNTP100

Deoxynucleotide Set, 100 mM

Xinhui Chen et al.
Biomedical chromatography : BMC, 31(3) (2016-08-25)
The endogenous deoxynucleoside triphosphate (dNTP) pool includes deoxyadenosine triphosphate (dATP), deoxycytidine triphosphate (dCTP), deoxyguanosine triphosphate (dGTP) and thymidine triphosphate (TTP). The endogenous dNTP pool is regulated by complex enzymatic pathways that can be targeted by drugs, such as antimetabolites. In
Michael G Mohsen et al.
Nucleic acids research, 47(18), 9495-9501 (2019-09-11)
We document the preparation and properties of dimerized pentaphosphate-bridged deoxynucleotides (dicaptides) that contain reactive components of two different nucleotides simultaneously. Importantly, dicaptides are found to be considerably more stable to hydrolysis than standard dNTPs. Steady-state kinetics studies show that the

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