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Merck
모든 사진(1)

문서

11051466001

Roche

dGTP

=98%, solution, 100 mM, suitable for PCR, suitable for DNA sequencing

동의어(들):

2′-Deoxyguanosine 5′-triphosphate trisodium salt solution, 2′-deoxy-guanosine-5′-triphosphate, deoxy-guanosine-5′-triphosphate, 2′-, dGTP-Na3

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About This Item

실험식(Hill 표기법):
C10H13N5Na3O13P3
CAS Number:
Molecular Weight:
573.13
MDL number:
UNSPSC 코드:
41116100
PubChem Substance ID:

Quality Level

설명

Lithium salt

분석

98%

형태

solution

사용

sufficient for 6,250 standard reactions (PCR)

포장

pkg of 250 μL (25 μmol; 100 mM)

제조업체/상표

Roche

농도

100 mM

기술

DNA amplification: suitable
DNA sequencing: suitable
DNA synthesis: suitable
PCR: suitable

저장 온도

−20°C

SMILES string

[Na+].[Na+].[Na+].NC1=Nc2c(ncn2[C@H]3C[C@H](O)[C@@H](COP([O-])(=O)OP([O-])(=O)OP(O)([O-])=O)O3)C(=O)N1

InChI

1S/C10H16N5O13P3.3Na/c11-10-13-8-7(9(17)14-10)12-3-15(8)6-1-4(16)5(26-6)2-25-30(21,22)28-31(23,24)27-29(18,19)20;;;/h3-6,16H,1-2H2,(H,21,22)(H,23,24)(H2,18,19,20)(H3,11,13,14,17);;;/q;3*+1/p-3/t4-,5+,6+;;;/m0.../s1

InChI key

IWGGLKOTEOCWQP-BIHLCPNHSA-K

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일반 설명

2′-Deoxyguanosine 5′-triphosphate (dGTP) is made of a guanine nucleobase attached to deoxyribose and a 5′-hydroxyl on the sugar linked to a chain of three phosphate residues. It is used by the cells for the synthesis of DNA with the help of DNA polymerase.
dGTP is a 100 mM clear, colorless solution of the lithium salt (pH 7).
dGTP solution in water (pH 7), 100 mM, lithium salt.

애플리케이션

2′-Deoxyguanosine 5′-triphosphate trisodium salt is utilized for methods such as DNA sequencing, PCR and other DNA polymerase based DNA synthesis and repair techniques.
Use dGTP, lithium salt, for DNA sequencing, labeling, and all types of amplification reactions or primer-extension reactions.

품질

Typical analysis:
  • ≥98% dGTP (HPLC), ≤1.5% dGDP (HPLC), ≤0.5% dGMP (HPLC)
  • Absorbance determination A250/A260, A280/A260, A290/A260
  • DNase activity negative, according to the current Quality Control procedures
  • Suitability for PCR

단위 정의

1 μmol dGTP = 13.7 A252 units

기타 정보

For life science research only. Not for use in diagnostic procedures.

Our PCR Grade deoxynucleotides are even purer than the lithium salts, to ensure excellent performance in your PCRs. PCR Grade deoxynucleotides contain the sodium salt of the nucleotide in PCR Grade Water.
For your convenience, some of our deoxynucleotides are also available in:
  • nucleotide sets (all 4 nucleotides in separate vials)
  • ready-to-use nucleotide mixes (premixed solutions of all 4 nucleotides)

They are also vital components of:
  • kits (dNTPs, buffer, and polymerase in separate vials)
  • ready-to-use mixes [polymerase, nucleotides, and buffer (premixed 2x solutions)]

Storage Class Code

12 - Non Combustible Liquids

WGK

nwg

Flash Point (°F)

No data available

Flash Point (°C)

No data available


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