추천 제품
생물학적 소스
human
재조합
expressed in yeast
분석
≥95% (SDS-PAGE)
형태
buffered aqueous solution
효능
>1x108 units/mg ED50
품질
endotoxin tested
특이 활성도
≥2 × 107 U/mg
분자량
~17.4 kDa
포장
pkg of 10 μg
저장 조건
avoid repeated freeze/thaw cycles
농도
0.1-10 mg/mL
기술
cell culture | mammalian: suitable
불순물
<10 EU/mLtested (LAL test)
색상
colorless
UniProt 수납 번호
배송 상태
dry ice
저장 온도
−20°C
유전자 정보
human ... TNF(7124)
유사한 제품을 찾으십니까? 방문 제품 비교 안내
일반 설명
TNFα (tumor necrosis factor α) belongs to the TNF superfamily and is mapped to human chromosome 6p21.33.
애플리케이션
Tumor Necrosis Factor-α human has been used to study the differential regulation of microRNA-146a , CFH (complement factor-H), IRAK-1 (interleukin-1 receptor associated kinase-1) and tetraspanin-12 in primary brain cell cultures in presence of TNF-α. To determine the intercellular adhesion molecule-1 (ICAM1) expression using ELISA.
생화학적/생리학적 작용
TNF-α is associated with formation, growth and destabilization of atherosclerotic plaques. TNF-α is involved in the disease progression of osteoarthritis.
Tumore necrosis factor-α (TNF-α), also known as cachectin, is expressed as a 26 kDa membrane bound protein and is then cleaved by TNF-α converting enzyme (TACE) to release the soluble 17 kDa monomer, which forms homotrimers in circulation. TNF-α plays roles in antitumor activity, immune modulation, inflammation, anorexia, cachexia, septic shock, viral replication and hematopoiesis. TNF-α is expressed by a great variety of cells, with numerous inductive and suppressive agents. Primarily, TNF-α is produced by macrophages in response to immunological challenges such as bacteria (lipopolysaccharides), viruses, parasites, mitogens and other cytokines. TNF-α is cytotoxic for many transformed cells (its namesake activity) but in normal diploid cells, it can stimulate proliferation (fibroblasts), differentiation (myeloid cells) or activation (neutrophils). TNF-α also shows antiviral effects against both DNA and RNA viruses and it induces production of several other cytokines. Although TNF-α is used in clinical trials as an antitumor agent, Sigma′s cytokine, growth factor and hormone products are for research only. TNF-α and the related molecule TNF-β (LT-α) share close structural homology with 28% amino acid sequence identity and both activate the same TNF receptors, TNFR1 and TNFR2. Mouse and human TNF-α share 79% amino acid sequence identity. Unlike human TNF-α, the mouse form is N-glycosylated.
물리적 형태
Solution in phosphate buffered saline containing 1 mg/ml bovine serum albumin.
분석 메모
The cytolytic activity of TNF-α against WEHI 164 cells has been measured in culture using an MTT toxicity assay. The ED50 is defined as the concentration of TNF-alpha that mediates half-maximal cytotoxicity in the presence of 1 micro-g/ml actinomycin D.
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 3
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
개인 보호 장비
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
이미 열람한 고객
Casper Steenholdt et al.
The American journal of gastroenterology, 109(7), 1055-1064 (2014-05-07)
Cost-effective guidance of therapeutic strategy in Crohn's disease patients with secondary infliximab (IFX) treatment failure may be achieved by serum IFX and anti-IFX antibody (Ab) measurements by radioimmunoassay (RIA). This study investigated implications of using other techniques for this purpose.
Fen Li et al.
Zhong nan da xue xue bao. Yi xue ban = Journal of Central South University. Medical sciences, 40(9), 945-954 (2015-09-27)
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Sirtuin1 (SIRT1) regulates central metabolic functions such as lipogenesis, protein synthesis, gluconeogenesis, and bile acid (BA) homeostasis through deacetylation. Here we describe that SIRT1 tightly controls the regenerative response of the liver. We performed partial hepatectomy (PH) to transgenic mice
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