The structure of the material after purification is not assessed. While the native folding state cannot be guaranteed, the final purified flagellan can bind human and mouse TLR5 and activate TLR5 in cell culture.
추천 제품
생물학적 소스
Salmonella enterica subsp. enterica serovar Typhimurium str. LT2
분석
>95% (SDS-PAGE)
양식
lyophilized
분자량
~50 kDa by SDS-PAGE
포장
pkg of 10 μg
불순물
<0.01 EU/μg endotoxin, tested
색상
white
UniProt 수납 번호
배송 상태
wet ice
저장 온도
−20°C
유전자 정보
Salmonella enterica subsp. enterica serovar Typhimurium str. LT2 ... fliC (STM1959)
일반 설명
FliC (flagellin) is the subunit protein which polymerizes to form the filaments of bacterial flagella. The protein has four-domains (D0, D1, D2, D3).[1]
The encoded flagellin filament contains 55 kDa monomers.[2]
생화학적/생리학적 작용
FliC (flagellin) can activate the innate immune system by TLR5 (toll like receptor 5) and is an important proinflammatory molecule.[1][3] FliC also induces the production of antibodies and is identified as an antigen by CD4+ T cells.[4]
It also stimulates the production of proinflammatory mediators : nitric oxide, interleukin 1 (IL-1), IL-6, IL-8, and tumor necrosis factor α.[5]
물리적 형태
Lyophilized from PBS.
재구성
Reconstitute with 100 μL deionized water.
Storage Class Code
10 - Combustible liquids
WGK
WGK 2
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
이미 열람한 고객
Mucosal administration of flagellin induces innate immunity in the mouse lung.
Honko A N and Mizel S B
Infection and Immunity, 72(11), 6676-6679 (2004)
In vivo, fliC expression by Salmonella enterica serovar Typhimurium is heterogeneous, regulated by ClpX, and anatomically restricted.
Cummings LA
Molecular Microbiology, 61, 795-809 (2006)
FliC-specific CD4+ T cell responses are restricted by bacterial regulation of antigen expression.
Cummings LA
Journal of Immunology, 174, 7929-7938 (2005)
Laís Rossi Perazza et al.
The Journal of nutrition, 150(10), 2673-2686 (2020-09-05)
Recent meta-analyses suggest that the consumption of fermented dairy products reduces type 2 diabetes and cardiovascular disease (CVD) risk, although the underlying mechanisms remain unclear. We evaluated whether dairy protein products modulated gut microbiota and cardiometabolic features in mouse models
Flagellin/TLR5 responses in epithelia reveal intertwined activation of inflammatory and apoptotic pathways.
Zeng H, et al.
American Journal of Physiology: Gastrointestinal and Liver Physiology, 290(1), G96-G108 (2006)
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Hello! I wondered if this product is expressed from the host in its native folded state? I am interested in obtaining native folded flagellin... thanks for any insights you can provide in this matter!
1 답변-
도움이 되었습니까?
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Good morning, I would like to have the sequence of this protein and the host where it was produced. Thank yo and best regards
1 답변-
This is native Flagellin purified from Salmonella typhimurium. The published sequence can be found here:
https://www.uniprot.org/uniprotkb/P06179/entry도움이 되었습니까?
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