SRP3196

EGF from mouse

recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture

• 동의어(들): Epidermal Growth Factor, URG, Urogastrone
• UNSPSC 코드: 12352202
• NACRES: NA.32

속성

• 생물학적 소스: mouse
• 재조합: expressed in E. coli
• 분석: ≥98% (HPLC); ≥98% (SDS-PAGE)
• 양식: lyophilized
• 효능: 0.1 ng/mL
• 분자량: 6.0 kDa
• 포장: pkg of 500 μg
• 기술: cell culture | mammalian: suitable
• 불순물: <0.1 EU/μg endotoxin, tested
• 색상: off-white to yellow
• UniProt 수납 번호: P01132
• 배송 상태: wet ice
• 저장 온도: −20°C
• 유전자 정보: mouse ... EGF(13645)

설명

일반 설명

EGF (epidermal growth factor) is a potent growth factor that stimulates the proliferation of various epidermal and epithelial cells.[1] Recombinant murine EGF is a 6kDa globular protein containing 53 amino acid residues, including 3 intramolecular disulfide-bonds.

애플리케이션

EGF from mouse has also been used in the DMEM/F12 media for growing mouse kidney cells.[2]

생화학적/생리학적 작용

EGF (epidermal growth factor) has been shown to inhibit gastric secretion, and to be involved in wound healing.[3][4] EGF signals through a receptor known as c-erbB, which is a class I tyrosine kinase receptor.[5] This receptor also binds with TGF (transforming growth factor)-α and VGF (vaccinia virus growth factor). EGF plays an important role in embryogenesis and cardiogenesis.[6][7]

서열

NSYPGCPSSY DGYCLNGGVC MHIESLDSYT CNCVIGYSGD RCQTRDLRWW ELR

물리적 형태

Lyophilized from 10 mM Sodium Phosphate, pH 8.2.

재구성

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.5-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

안전성 정보

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable