SRP3155

SLIT-2-N human

recombinant, expressed in HEK 293 cells, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture

• 동의어(들): SLIL3, Slit homolog 2 protein
• UNSPSC 코드: 12352202
• NACRES: NA.32

속성

• 생물학적 소스: human
• 재조합: expressed in HEK 293 cells
• 분석: ≥98% (HPLC); ≥98% (SDS-PAGE)
• 양식: lyophilized
• 분자량: 120.0-140.0 kDa
• 포장: pkg of 200 μg; pkg of 25 μg
• 기술: cell culture | mammalian: suitable
• 불순물: <0.1 EU/μg endotoxin, tested
• 색상: white
• UniProt 수납 번호: O94813
• 배송 상태: wet ice
• 저장 온도: −20°C
• 유전자 정보: human ... SLIT2(9353)

설명

일반 설명

Slit guidance ligand 2 (SLIT-2) is a member of the Slit family that signals through the Roundabout (Robo) receptor.[1] It is also referred to as SLIL3. SLIT2 is expressed primarily in the fetal lung, kidney, and adult spinal cord, and to a lesser extent in adult adrenal gland, thyroid and trachea. It is initially synthesized as a 1499 amino acid precursor, which is subsequently cleaved into N-terminal and C-terminal fragments, designated as Slit2-N and Slit2-C respectively. The neurodevelopment related activities, as measured by the ability to repel olfactory bulb axons and to induce branching in dorsal root ganglia axons, are contained only in the N-terminal fragment. Recombinant human Slit2-N is a 1093 amino acid glycoprotein corresponding to the N-terminal portion of the full length Slit2 precursor. Due to glycosylation Slit2-N migrates at an apparent molecular weight of approximately 120.0-140.0kDa by SDS-PAGE analysis under reducing conditions.

애플리케이션

Slit guidance ligand 2 (SLIT-2) has been used to treat cells in podocyte culture and in myosin II regulatory light chain (MRLC) phosphorylation assay.[2]

생화학적/생리학적 작용

Slit guidance ligand 2 (SLIT-2) acts as a repellent for axon guidance and neuronal migration, and it can also act as a chemoattractant to vascular endothelial cells and a chemotaxis inhibitor for leukocytes. It prevents the migration of T cells, neutrophils and macrophages, but enhances eosinophil migration. The protein has been shown to be downregulated in cancers.[3][4][1]

서열

QACPAQCSCS GSTVDCHGLA LRSVPRNIPR NTERLDLNGN NITRITKTDF AGLRHLRVLQ LMENKISTIE RGAFQDLKEL ERLRLNRNHL QLFPELLFLG TAKLYRLDLS ENQIQAIPRK AFRGAVDIKN LQLDYNQISC IEDGAFRALR DLEVLTLNNN NITRLSVASF NHMPKLRTFR LHSNNLYCDC HLAWLSDWLR QRPRVGLYTQ CMGPSHLRGH NVAEVQKREF VCSGHQSFMA PSCSVLHCPA ACTCSNNIVD CRGKGLTEIP TNLPETITEI RLEQNTIKVI PPGAFSPYKK LRRIDLSNNQ ISELAPDAFQ GLRSLNSLVL YGNKITELPK SLFEGLFSLQ LLLLNANKIN CLRVDAFQDL HNLNLLSLYD NKLQTIAKGT FSPLRAIQTM HLAQNPFICD CHLKWLADYL HTNPIETSGA RCTSPRRLAN KRIGQIKSKK FRCSAKEQYF IPGTEDYRSK LSGDCFADLA CPEKCRCEGT TVDCSNQKLN KIPEHIPQYT AELRLNNNEF TVLEATGIFK KLPQLRKINF SNNKITDIEE GAFEGASGVN EILLTSNRLE NVQHKMFKGL ESLKTLMLRS NRITCVGNDS FIGLSSVRLL SLYDNQITTV APGAFDTLHS LSTLNLLANP FNCNCYLAWL GEWLRKKRIV TGNPRCQKPY FLKEIPIQDV AIQDFTCDDG NDDNSCSPLS RCPTECTCLD TVVRCSNKGL KVLPKGIPRD VTELYLDGNQ FTLVPKELSN YKHLTLIDLS NNRISTLSNQ SFSNMTQLLT LILSYNRLRC IPPRTFDGLK SLRLLSLHGN DISVVPEGAF NDLSALSHLA IGANPLYCDC NMQWLSDWVK SEYKEPGIAR CAGPGEMADK LLLTTPSKKF TCQGPVDVNI LAKCNPCLSN PCKNDGTCNS DPVDFYRCTC PYGFKGQDCD VPIHACISNP CKHGGTCHLK EGEEDGFWCI CADGFEGENC EVNVDDCEDN DCENNSTCVD GINNYTCLCP PEYTGELCEE KLDFCAQDLN PCQHDSKCIL TPKGFKCDCT PGYVGEHCDI DFDDCQDNKC KNGAHCTDAV NGYTCICPEG YSGLFCEFSP PMV

물리적 형태

Lyophilized from 20 mM Tris + 200 mM L-Arginine, pH 8.8.

재구성

Centrifuge the vial prior to opening. Reconstitute in 20mM Tris, pH 8.8 + 150mM NaCl to a concentration of 0.1-1.0mg/ml. Note: Slow to dissolve. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

안전성 정보

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable