SRP3085

Interleukin-2, human

Animal-component free, recombinant, expressed in E. coli, suitable for cell culture

• 동의어(들): Aldesleukin, IL-2, T-cell growth factor (TCGF)
• UNSPSC 코드: 12352202
• NACRES: NA.32

속성

• 생물학적 소스: human
• 재조합: expressed in E. coli
• 분석: ≥95% (HPLC)
• 양식: (Lyophilized with no additives from a 0.2µ filtered solution)
• 효능: ≤0.5 ng/mL ED₅₀
• 분자량: 15.5 kDa
• 포장: pkg of 50 μg
• 기술: cell culture | mammalian: suitable
• 불순물: ≤1.00 EU/μg endotoxin, tested
• UniProt 수납 번호: P60568
• 배송 상태: wet ice
• 저장 온도: −20°C
• 유전자 정보: human ... IL2(3558)

설명

일반 설명

IL-2 (interleukin 2) is a powerful immunoregulatory lymphokine produced by T-cells in response to antigenic or mitogenic stimulation.[1][2] It is a type I cytokine.[3] The gene is mapped to human chromosome 4q27.[4] Recombinant human IL-2 is a 15.5kDa protein, containing 134 amino acid residues including one intrachain disulfide bond.

애플리케이션

• IL-2 (interleukin 2) human has been used for the detection of IL-2 using 4-Fluoro-3-nitrophenyl grafted gold electrode based platform.[5]
• It has been used for the production of tumor-specific cytotoxic T lymphocytes.[6]
• It has been used for the proliferation of T lymphocytes.[7]

생화학적/생리학적 작용

IL-2/IL-2R (interleukin 2/IL-2 receptor) signaling is required for T-cell proliferation and other fundamental functions which are essential for the immune response. IL-2 stimulates growth and differentiation of B-cells, NK (natural killer) cells, lymphokine activated killer cells, monocytes, macrophages and oligodendrocytes.[1][8] It is associated with recurrent oral ulcer and asthma and Mycoplasma pneumoniae infection in children.[9][10] Absence of IL2 in mice causes fatal lymphoproliferative inflammatory disease (IL-2 deficiency syndrome).[11] IL-2 signaling involves three pathways: phosphoinositol 3-kinase (PI3-K)/AKT, Ras-MAPK (mitogen activated protein kinase), and JAK-STAT (Janus kinase - signal transducers and activators of transcription) pathways.[3]

서열

MAPTSSSTKK TQLQLEHLLL DLQMILNGIN NYKNPKLTRM LTFKFYMPKK ATELKHLQCL EEELKPLEEV LNLAQSKNFH LRPRDLISNI NVIVLELKGS ETTFMCEYAD ETATIVEFLN RWITFAQSII STLT

물리적 형태

Lyophilized with no additives.

재구성

Reconstitute the contents of the vial using 100 mM acetic acid to a concentration of 0.1-1.0 mg/mL. This solution can then be diluted into other aqueous buffers and stored at 2-8 °C for up to 1 week. For extended storage, freeze in working aliquots. It may be advisable to centrifuge the vial prior to reconstitution.

안전성 정보

• 픽토그램: GHS07
• 신호어: Warning
• 유해 및 위험 성명서: H319,H335
• 예방조치 성명서: P261 - P264 - P271 - P280 - P304 + P340 + P312 - P305 + P351 + P338
• Hazard Classifications: Eye Irrit. 2 - STOT SE 3
• 표적 기관: Respiratory system
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1