A superior substrate than biotinyl tyramide (biotin-phenol; BP) for APEX2-mediated proximity-labeling of RNA.
Biotin-aniline (Btn-An) is a superior substrate than biotinyl tyramide (biotin-phenol; BP) for APEX2-mediated proximity labeling of RNA (∼25-fold higher by dot blot with 0.5 mM substrate and 0.4-10 μg RNA/dot). Btn-An specifically labels mitochondrial, but not cytoplasmic, mRNAs in live HEK293T stable line expressesing APEX2 in the mitochondrial matrix (mito-APEX2).
Angewandte Chemie (International ed. in English), 58(34), 11763-11767 (2019-06-27)
The subcellular organization of biomolecules such as proteins and nucleic acids is intimately linked to their biological functions. APEX2, an engineered ascorbate peroxidase that enables proximity-dependent labeling of proteins in living cells, has emerged as a powerful tool for deciphering
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