SAB5801017

Alpaca Anti-Mouse kappa HRP single domain Antibody

recombinant Alpaca(VHH) Nano antibody

• 동의어(들): Recombinant Alpaca single variable domain secondary Nanobody
• UNSPSC 코드: 12352203
• NACRES: NA.41

속성

• 생물학적 소스: alpaca
• 재조합: expressed in E. coli
• 결합: horse radish peroxidase (HRP)
• 항체 형태: affinity purified immunoglobulin
• 항체 생산 유형: secondary antibodies
• 클론: recombinant monoclonal
• 양식: lyophilized solid
• 분자량: 14 kDa
• 종 반응성: mouse, Alpaca
• 기술: ELISA: 1:5000-1:20000; western blot: 1:5000-1:20000
• 크기: 0.1 mL
• 색상: colorless
• 동형: VHH
• 배송 상태: wet ice
• 저장 온도: 2-8°C

설명

일반 설명

Alpaca Anti-Mouse kappa HRP single domain Antibody is designed to specifically detect mouse IgG kappa chain. It is a monovalent, single domain, recombinant antibody to mouse IgG kappa chain coupled to HRP. Based on immunoelectrophoresis and/or ELISA, Alpaca Anti-Mouse kappa HRP single domain Antibody detects the mouse IgG kappa chain selectively but not with the mouse IgG lambda chain or heavy chain of mouse IgG.

Most monoclonal antibodies are generated in mouse. There are five antibody isotypes (IgA, IgD, IgE, IgG, and IgM) from mouse. Each isotype has a different heavy chain. Mouse IgG constitutes 75% of serum immunoglobulins, and IgG is the predominant form of first antibody produced from mouse. Mouse IgG consists of five subclasses-IgG1, IgG2a, IgG2b, IgG2c(inbred mouse strains with the Igh1-b allele have IgG2c isotype instead of IgG2a), IgG3. They are highly homologous and differ mainly in the hinge region. The whole IgG molecule possesses both the Fc region and the Fab region, which possessing the epitope-recognition site. The IgG contains two heavy and light chains(kappa or lambda), and the heavy chain is about 50 KD and the light chain is about 25 KD. The common IgG is monomeric with a molecular weight of approximately 150 kD.
VHH are single-domain antibodies derived from the variable regions of heavy chain of Camelidae immunoglobulin. The size of VHH is extremely small(<15KDa) compared to other forms of antibody fragment, which significantly increase the permeability of VHH. Thus VHH is considered of great value for research, diagnostics and therapeutics.

특이성

Mouse IgG kappa chain
Isotype: VHH domain of alpaca IgG2b/2c

면역원

Recombinant Alpaca single variable domain secondary nano antibody

포장

100 μg in Glass bottle

물리적 형태

Lyophilized from 10mM PBS (pH 7.5), 0.05% sucrose, 0.1% trehalose

재구성

Add 100uL dH2O or 100uL dH2O with 50% Glycerol to a final concentration of 1mg/mL

저장 및 안정성

After reconstitution, store at –20 °C(Avoid freeze / thaw cycles). Protect from light. Stable for 12 months at -20°C

기타 정보

No cross-reactivity with rabbit, human, cynomolgus, rat, goat IgG

안전성 정보

• 픽토그램: GHS07
• 신호어: Warning
• 유해 및 위험 성명서: H317,H412
• 예방조치 성명서: P261 - P272 - P273 - P280 - P302 + P352 - P333 + P313
• Hazard Classifications: Aquatic Chronic 3 - Skin Sens. 1
• Storage Class Code: 13 - Non Combustible Solids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable