SAB4200826

Anti- Cholera Toxin-Peroxidase antibody produced in rabbit

IgG fraction of antiserum

• 동의어(들): Cholera enterotoxin subunit B (ctxB), Cholera enterotoxin, A chain (ctxA)
• UNSPSC 코드: 12352203
• NACRES: NA.41

속성

• 생물학적 소스: rabbit
• 결합: peroxidase conjugate
• 항체 형태: IgG fraction of antiserum
• 항체 생산 유형: primary antibodies
• 클론: polyclonal
• 설명: Research area: Microbiome
• 양식: lyophilized powder
• 분자량: subunit mol wt ~12 kDa (non-toxic CTxB ); subunit mol wt ~27 kDa (the toxic CTxA )
• 종 반응성: Vibrio cholerae
• 농도: ~4 mg/mL
• 기술: direct ELISA: 1:8,000-1:16,000 using 5 μg/ml Cholera Toxin for coating
• UniProt 수납 번호: P01555 (ctxA); P01556 (ctxB)
• 배송 상태: dry ice
• 저장 온도: −20°C
• 타겟 번역 후 변형: unmodified
• 유전자 정보: Vibrio cholerae ... ctxA(57740120) , ctxB(57740119)

설명

일반 설명

Cholera toxin (CTx) also known as choleragen, is an enterotoxin produced by the Gram-negative bacterium Vibrio cholerae that naturally habitat in fresh or saltwater environments. CTx is composed of two subunits, the toxic CTxA (~27 kDa) and non-toxic CTxB (~12 kDa) assembled with the stoichiometry AB5. The V. cholerae virulence factors CtxA and CtxB are located at the CTX phage genome integrated within the bacterial chromosome.

특이성

Anti-Cholera Toxin-peroxidase antibody specifically recognizes Cholera Toxin and has no cross reactivity with Staphylococcal Enterotoxin A (SEA), Staphylococcal Enterotoxin B (SEB) or Pseudomonas Exotoxin A

면역원

purified toxin from Vibrio cholera

애플리케이션

Anti- Cholera Toxin-Peroxidase antibody produced in rabbit has been used in direct enzyme linked immunosorbent assay.

생화학적/생리학적 작용

The A1 fragment of the cholera toxin (CTx)A subunit is translocated through the membrane of the host cell, where it catalyses the ADP-ribosylation of the α-subunit of Gs (Gsa), a regulatory component of the adenylate cyclase complex. The resulting increased level of cyclic AMP promotes a wide variety of actions, including the secretion of chloride ions in the case of intestinal epithelial cells. Most of the V. cholerae species do not cause any disease in human, but few including serotypes O1 and O139 can cause cholera pandemics. Antibodies specific for cholera toxin may be used in studies of structural and functional aspects of toxin membrane interactions and for the detection of CTxB, when used, for example, as an adjuvant when injected mucosally together with the desired antigen.

물리적 형태

Supplied as a lyophilized powder

저장 및 안정성

Store the lyophilized product at 2–8 °C. For extended storage after reconstitution, keep at –20 °C in working aliquots. Avoid repeated freeze-thaw cycles. For continuous use after reconstitution, keep at 2–8 C for up to 1 month. Solutions at working dilution should be discarded if not used within 12 hours.

안전성 정보

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 2
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable