SAB4200337

Anti-phospho-RasGRP1(pThr¹⁸⁴) antibody, Mouse monoclonal

clone p-RasGRP1-1, purified from hybridoma cell culture

• 동의어(들): Anti-CALDAG-GEFI, Anti-CALDAG-GEFII, Anti-RAS guanyl releasing protein 1 (calcium and DAG-regulated) hRasGRP1
• UNSPSC 코드: 12352203
• NACRES: NA.41

속성

• 생물학적 소스: mouse
• Quality Level: 200
• 결합: unconjugated
• 항체 형태: purified from hybridoma cell culture
• 항체 생산 유형: primary antibodies
• 클론: p-RasGRP1-1, monoclonal
• 양식: buffered aqueous solution
• 분자량: antigen ~90 kDa
• 종 반응성: human
• 농도: ~1.0 mg/mL
• 기술: immunoprecipitation (IP): suitable; western blot: 2-4 μg/mL using whole extracts of HEK-293T cells overexpressing human RasGRP1
• 동형: IgG2a
• UniProt 수납 번호: O95267
• 배송 상태: dry ice
• 저장 온도: −20°C
• 타겟 번역 후 변형: phosphorylation (pThr184)
• 유전자 정보: human ... RASGRP1(10125) ; mouse ... Rasgrp1(19419) ; rat ... Rasgrp1(29434)

설명

일반 설명

Monoclonal Anti-phospho-RasGRP1(pThr184) (mouse IgG2a isotype) is derived from the hybridoma p-RasGRP1-1 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic phospho-peptide corresponding to residues surrounding Thr184 of human RasGRP1 conjugated to KLH. RAS guanyl releasing protein 1 (RasGRP1) is a member of RasGRP family, characterized by the presence of a N-terminal Ras superfamily guanine nucleotide exchange factor (GEF) domain and and C-terminal calcium- and diacylglycerol (DAG)/phorbol ester-binding domains. It is an intracellular signaling protein. RasGRP1 has a N terminal guanine nucleotide exchange factor domain for Ras-like guanosine triphosphatases (GTPases) (RasGEFN/RasGEF), two EF hand motifs and a carboxy-terminal C1 domain.

면역원

a synthetic phospho-peptide corresponding to residues surrounding Thr¹⁸⁴ of human RasGRP1, conjugated to KLH. The corresponding sequence is identical in rat, mouse, monkey, bovine and canine RasGRP1.

애플리케이션

Monoclonal Anti-phospho-RasGRP1(pThr184) antibody produced in mouse has been used in immunoblotting and immunoprecipitation.

생화학적/생리학적 작용

RAS guanyl releasing protein 1 (RasGRP1) functions as a diacylglycerol (DAG)-regulated nucleotide exchange factor specifically activating Ras through the exchange of bound guanosine diphosphate (GDP) for guanosine triphosphate (GTP). It activates the extracellular signal-regulated kinase (Erk)/mitogen-activated protein (MAP) kinase cascade and regulates T-cells and B-cells development, homeostasis and differentiation.

물리적 형태

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 10 - Combustible liquids
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable