S2076

α-2,6-Sialyltransferase from Photobacterium damsela

recombinant, expressed in E. coli BL21, ≥5 units/mg protein

• 동의어(들): β-Galactoside α-2,6-sialyltransferase, CMP-N-Acetylneuraminate:β-D-galactosyl-1,4-N-acetyl-β-D-glucosamine α-2,6-N-acetylneuraminyltransferase
• 효소 위원회 번호: 2.4.99.1 (BRENDA, IUBMB)
• MDL number: MFCD00132245
• UNSPSC 코드: 12352204
• NACRES: NA.54

속성

• 재조합: expressed in E. coli BL21
• Quality Level: 200
• 양식: lyophilized powder
• 특이 활성도: ≥5 units/mg protein
• 분자량: 56.8 kDa
• 배송 상태: dry ice
• 저장 온도: −20°C

설명

일반 설명

Human ST6Gal-I (β-galactoside α-2,6-sialyltransferase 1) is a member of the CAZy family GT29.[1]

애플리케이션

α-2,6-Sialyltransferase from Photobacterium damsela has been used in resialylation and restoration of sialic acids (SAs) in HRT-18G cells.[2]

Highly active α2-6 sialyltransferase has been used to prepare high levels of disialylated fragment crystals. [3]

생화학적/생리학적 작용

Sialyltransferase transfers Neu5Ac from CMP-Neu5Ac to the galactosyl terminus of acceptor molecules including glycoproteins, glycolipids, and oligosaccharides.

The terminal step of complex N-glycan biosynthesis is catalysed by α-2,6-sialyltransferase (STs).[1] Bacterial α(2,6)-STs possesses broader acceptor substrate specificity when compared to eukaryotic α(2,6)-STs.[4]

단위 정의

One unit will catalyze the formation of 1 μmol Neu-5-Ac-α-2,6-LacMU from CMP-Neu-5-Ac and Lac-β−OMU per minute at 37 °C at pH 8.0.

물리적 형태

Supplied as a lyophilized powder containing Tris-HCl and NaCl.

분석 메모

Enzymatic activity assays are performed in Tris-HCl buffer (100 mM, pH 8.0) containing CMP-Neu-5-Ac (1 mM) and Lac-β−OMU (1 mM) at 37 °C for 30 min and analyzed using HPLC with a fluorescence detector (excitation at 325 nm and emission at 372 nm).

안전성 정보

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable