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Merck
모든 사진(1)

주요 문서

S2014

Sigma-Aldrich

Staphylococcus aureus

buffered aqueous suspension, Wood 46 strain

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About This Item

UNSPSC 코드:
12352202
NACRES:
NA.81
가격 및 재고 정보를 현재 이용할 수 없음 고객지원팀으로 연락바랍니다.

생물학적 소스

Staphylococcus aureus

무균

Not processed or packaged aseptically

양식

buffered aqueous suspension

구성

Cell suspension, ~10% wet weight/volume

저장 온도

2-8°C

일반 설명

Staphylococcus aureus Wood 46 is protein A deficient and spa negative.[1] It shares 98% to 99% genome identity with S.aureus[1] and shows a lower surface expression of cell wall-associated protein A.[2]

애플리케이션

(Not intended for use as a starter culture.)
Staphylococcus aureus has been used:
  • to mimic infection and induce fever in Pekin duck[3]
  • to test its effect on hemocyte morphology in hemolymph samples from beetle Tenebrio molitor larva[4]
  • in the antibacterial activity and minimum inhibitory concentration (MIC) assay with gedunin and 7-deacetoxy-7αhydroxygedunin potassium salt [5]

Wood 46, a non-protein A producing S. aureus strain[6], prepared by the same method as P7155 (Protein A, crude cell suspension-Cowan strain), may be used as a control in protein A-immunoglobulin binding studies.

생화학적/생리학적 작용

Staphylococcus aureus Wood 46 displays reduced virulence compared to the S. aureus.[1] This isolate is useful in understanding protein A role in pathogenesis and virulence.[2]

물리적 형태

Formalin-fixed crude cell suspension of essentially non-viable S. aureus (Wood 46 strain) in 0.05 M potassium phosphate buffer, pH 7.5, containing 0.2% sodium azide

제조 메모

Produced in pure culture.

분석 메모

This strain binds less than 10% of the rabbit IgG bound by P 7155 as assayed by a modification of the method of Kessler[7].

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable


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문서 라이브러리 방문

M Marais et al.
Poultry science, 90(6), 1234-1238 (2011-05-21)
Poultry, like mammals and other birds, develop fever when exposed to compounds from gram-negative bacteria. Mammals also develop fever when exposed to the constituents of viruses or gram-positive bacteria, and the fevers stimulated by these different pathogenic classes have discrete
G Kronvall et al.
Infection and immunity, 3(1), 10-15 (1971-01-01)
Protein A of Staphylococcus aureus can be detected on cell walls of intact bacteria by use of radioactively labeled myeloma globulin. Of 156 strains of S. aureus, 141 (90%) contained protein A. None of 47 S. epidermidis strains was positive
Synthetic modification of gedunin and comparative antibacterial activity of gedunin and 7-deacetoxy-7alpha-hydroxygedunin potassium salt
Okhale SE, et al.
African Journal of Pure and Applied Chemistry, 6(14), 183-189 (2012)
S W Kessler
Journal of immunology (Baltimore, Md. : 1950), 117(5 Pt 1), 1482-1490 (1976-11-01)
Procedures are detailed for the rapid isolation of representative cell membrane antigens with protein A-bearing staphylococci as an adsorbent for IgG antibodies complexed with the antigens. Cell surface membrane proteins were radioiodinated and solubilized in nonionic detergent. Specific antisera were
Manasi Balachandran et al.
Genome announcements, 5(13) (2017-04-01)
Here, we report the first complete genome sequence of the Staphylococcus aureus strain Wood 46. Wood 46 has played an important role in understanding the virulence and pathogenesis of S. aureus infections. This report will assist efforts in vaccine development against

질문

  1. Hello, I'm wondering if this "Staphylococcus aureus, wood 46 strain" is deactivated or not. Additionally, do you have the corresponding aptamer or antibody of this strain?

    1 답변
    1. This product is a formalin-fixed crude cell suspension of essentially non-viable S. aureus in 0.05 M potassium phosphate buffer containing 0.2% sodium azide. Currently, there is no corresponding aptamer or antibody for the Wood 46 Strain.

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