R7904

Anti-Rab5 antibody, Mouse monoclonal

clone Rab5-65, purified from hybridoma cell culture

• 동의어(들): Anti-RAS-associated Protein RAB5A
• MDL number: MFCD01322589
• UNSPSC 코드: 12352203
• NACRES: NA.41

속성

• 생물학적 소스: mouse
• 결합: unconjugated
• 항체 형태: purified immunoglobulin
• 항체 생산 유형: primary antibodies
• 클론: Rab5-65, monoclonal
• 양식: buffered aqueous solution
• 분자량: ~24 kDa
• 종 반응성: human, cat, bovine, mouse, rat
• 농도: ~2 mg/mL
• 기술: immunocytochemistry: 5-10 μg/mL using HeLa cells or NIH-3T3 or NRK; indirect ELISA: suitable; microarray: suitable; western blot: suitable
• 동형: IgG3
• UniProt 수납 번호: P20339
• 배송 상태: dry ice
• 저장 온도: −20°C
• 타겟 번역 후 변형: unmodified
• 유전자 정보: human ... RAB5A(5868) ; mouse ... Rab5a(271457) ; rat ... Rab5a(64633)

설명

일반 설명

Anti-Rab5 antibody, Mouse monoclonal (mouse IgG3 isotype) is derived from the hybridoma Rab5-65 produced by the fusion of mouse myeloma cells (NS1 cells) and splenocytes from BALB/c mice immunized with a synthetic peptide corresponding to C-terminal region of human Rab5, conjugated to KLH. Rab5 is ubiquitously expressed in human tissues. It localizes mainly to early endosomes, but is also present on the plasma membrane.

Rab5 (Ras-related protein Rab-5A) is a small GTPase , that has three isoforms - Rab5A, Rab5B and Rab5C. It is upregulated in several secretory cells. It is located on human chromosome 3p24.

면역원

synthetic peptide corresponding to the C- terminal region of human Rab5, conjugated to KLH.

애플리케이션

Anti-Rab5 antibody, Mouse monoclonal has been used in:

• enzyme linked immunosorbent assay (ELISA)
• immunocytochemistry
• co-immunoprecipitation assay
• western blotting
• immunofluorescence

생화학적/생리학적 작용

Among the proteins recruited by the GTP-bound active Rab5 are Rabaptin-5 and early endosome antigen 1(EEA1).

Rab5 (Ras-related protein Rab-5A) controls endosomal trafficking. Overexpression of Rab5A leads to metastasis and poor prediction in colorectal cancer patients. It modulates common Rab5 effectors to control the secretory pathways. It controls the homotypic integration between early endosomes and also the integration between endocytic vesicles and early endosomes.

물리적 형태

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)