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Merck
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Key Documents

R4526

Sigma-Aldrich

Reference Dye for Quantitative PCR

100 ×, solution

동의어(들):

Reference dye for qPCR, ROX

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About This Item

UNSPSC 코드:
41106300
NACRES:
NA.55

형태

solution

Quality Level

사용

sufficient for ≥600 reactions

농도

100 ×

기술

PCR: suitable

색상

red

solubility

water: soluble

저장 온도

2-8°C

일반 설명

Sigma′s Reference Dye for quantitative polymerase chain reaction (qPCR) is a proprietary dye for use with real-time PCR. It is used for the normalization of reaction data when using SYBR Green, molecular probes, or dual-labeled probe chemistries for real-time detection. The Reference Dye is supplied as a 100× solution with a maximum excitation and emission of 586 nm and 605 nm, respectively. Instrument settings for ROX reference dye are satisfactory for the measurement of Reference Dye for qPCR.

애플리케이션

Reference Dye for Quantitative PCR has been used:
  • in the preparation of mastermix for real time-quantitative polymerase chain reaction (RT-qPCR)
  • as a component of the reaction mixture for detection of Clostridium difficile by quantitative polymerase chain reaction (qPCR)
  • for analyzing the degree of cellular DNA contamination by qPCR

기타 정보

Reference Dye for Quantitative PCR is forR&D use only. Not for drug, household, or other uses.

픽토그램

Exclamation markEnvironment

신호어

Warning

유해 및 위험 성명서

Hazard Classifications

Aquatic Acute 1 - Aquatic Chronic 1 - Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

개인 보호 장비

Eyeshields, Gloves


시험 성적서(COA)

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프로토콜

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