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PKH67GL

Sigma-Aldrich

PKH67 Green Fluorescent Cell Linker Kit for General Cell Membrane Labeling

Distributed for Phanos Technologies

동의어(들):

Green PKH membrane labeling kit

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크기 선택

1 KIT
₩2,003,509

₩2,003,509

출고 가능일2025년 4월 16일세부사항

모든 사진(2)

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1 KIT
₩2,003,509

About This Item

UNSPSC 코드:
12352207
NACRES:
NA.32

₩2,003,509

출고 가능일2025년 4월 16일세부사항

Quality Level

200

포장

pkg of 1 kit

저장 조건

protect from light

형광

λex 490 nm; λem 502 nm (PKH67 dye)

검출 방법

fluorometric

배송 상태

ambient

저장 온도

room temp

관련 카테고리

애플리케이션

PKH67 Green Fluorescent Cell Linker Kit for General Cell Membrane Labeling has been used:
  • To label and further investigate the exosomes expelled from cells in triple-negative breast cancer condition.[1]
  • To label apoptotic cells,[2] in order to study the role of ανβ5 receptor in both binding and internalization of apoptotic cells.[3]
  • In fluorescence imaging.[4]

This kit is for general cell membrane labeling. PKH67 has a longer aliphatic carbon tail than PKH1 and PKH2, two other green dyes previously described for in vitro and in vivo cell tracking. Based on the longer tail length, in-house studies have consistently shown reduced cell-cell transfer for PKH67 as compared to PKH2.
Slow loss of fluorescence has been observed in in vivo studies using PKH1 and PKH2. PKH67 may exhibit similar properties since this behavior appears to be characteristic of green cell linker dyes, but not red cell linker dyes. Correlation of in vitro cell membrane retention with in vivo fluorescence half life in non-dividing cells predicts an in vivo fluorescence half life of 10-12 days for PKH67. Other green cell linker dyes with similar half lives have been used to monitor in vivo lymphocyte and macrophage trafficking over periods of 1-2 months, suggesting that PKH67 will also be useful for in vivo tracking studies of moderate length.

결합

For additional technical details on PKH and CellVue® Fluorescent Cell Linker Dyes including an extensive bibliography, please visit here.

법적 정보

CellVue is a registered trademark of Phanos Technologies

키트 구성품 전용

제품 번호
설명
  • Diluent C 6 x 10
  • PKH67 Cell Linker in ethanol .5 mL

픽토그램

FlameExclamation mark
GHS02,GHS07

신호어

Danger

유해 및 위험 성명서

H225,H319

Hazard Classifications

Eye Irrit. 2 - Flam. Liq. 2

Storage Class Code

3 - Flammable liquids

Flash Point (°F)

57.2 °F - closed cup

Flash Point (°C)

14 °C - closed cup

가장 최신 버전 중 하나를 선택하세요:

시험 성적서(COA)

Lot/Batch Number
MKCV4102
MKCQ9079
MKCP3216
MKCQ0194
MKCK9600

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특정 버전이 필요한 경우 로트 번호나 배치 번호로 특정 인증서를 찾을 수 있습니다.

COA 검색

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Sucrose ≥99.5% (GC), BioReagent, suitable for cell culture, suitable for insect cell culture

Sigma-Aldrich

S1888

Sucrose

3,3′-Dioctadecyloxacarbocyanine perchlorate

Sigma-Aldrich

D4292

3,3′-Dioctadecyloxacarbocyanine perchlorate

Anjali P Kusumbe et al.
Stem cells (Dayton, Ohio), 27(3), 498-508 (2009-03-04)
Recruitment and localization of endothelial precursors within tumors is a potential area for the development of therapeutics, because their functional contribution to tumor vasculature is realized to be important for cancer cell survival. However, the exact nature of the recruited
Exosomes from triple-negative breast cancer cells can transfer phenotypic traits representing their cells of origin to secondary cells
O Brien K, et al.
European Journal of Cancer, 49(8), 1845-1859 (2013)
Hybrid microscaffold-based 3D bioprinting of multi-cellular constructs with high compressive strength: A new biofabrication strategy
Yu Jun T, et al.
Scientific Reports, 6, 39140-39140 (2016)
Dandan Ma et al.
Cells, 9(4) (2020-04-01)
Human salivary histatin 1 (Hst1) and Hst2 exhibit a series of cell-activating properties (e.g., promoting adhesion, spreading, migration and metabolic activity of mammalian cells). In contrast, Hst5 shows an anti-fungal property but no cell-activating properties. Previous findings suggest that their
Increased expression of inducible HSP70 in apoptotic cells is correlated with their efficacy for antitumor vaccine therapy
Masse D, et al.
International Journal of Cancer. Journal International Du Cancer, 111(4), 575-583 (2004)
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문서

PKH Linker Kits for Fluorescent Cell Labeling

PKH dyes are easy to use and achieve stable, uniform, and reproducible fluorescent labeling of live cells. PKH dyes are non-toxic membrane stains which produce high signal to noise ratio.

Lipophilic Dye Kits for Cell Tracking

Lipophilic cell tracking dyes enable cancer biologists to track tumor and immune cell functions both in vitro and in vivo. Read the article to choose a right membrane dye kit for cell tracking and proliferation monitoring.

Cell Tracking with Lipophilic Membrane Dyes

Optimal staining is a key component for studying tumorigenesis and progression. Learn useful tips and techniques for dye applications, including examples from recent studies.

PKH and CellVue® Claret Fluorescent Cell Linker Dyes

PKH and CellVue® Fluorescent Cell Linker Kits provide fluorescent labeling of live cells over an extended period of time, with no apparent toxic effects.

질문

1–10 / 12 질문  

  1. I mark mitochondria for injection with PKH26 Red Fluorescent Cell Linker Kit for General Cell Membrane Labeling. I want to use another dye to mark another type of mitochondria and inject them together in the same fish egg. Is PKH67 ok for this like PKH26?

    1 답변

    1. Yes, Product PKH67 would be suitable for this application. In this paper, both Products PKH26 and PKH67 are used in a similar application:
      https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5679712/

      도움이 되었습니까?

  2. What can be the alternative for removing excess dye after labeling of exosomes using PKH67 dye apart from ultracentrifugation?

    1 답변

    1. Excess dye is bound to serum or albumin which is added to the cell/dye mixture as a stop reaction. The recommended clean up or dye removal is by standard centrifugation (400 x g for 10 minutes, wash, and repeat). See the Product Information Sheet, page 3, bullet point 8 for more information:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/984/984/pkh67glbul.pdf

      Alternate methods for excess dye removal have not been investigated.

      도움이 되었습니까?

  3. Can we remove the excess PKH67 DYE used for the labeling of exosomes using exosome spin column rather than ultracentrifugation.

    1 답변

    1. The recommended exosome labeling protocol has been optimized to assure the best possible results. The use of exosome spin columns with the PKH and CellVue products has not been validated. See below for a link to our exosome labeling protocol. A spin column method may not offer the best results.

      https://www.sigmaaldrich.com/technical-documents/protocol/cell-culture-and-cell-culture-analysis/imaging-analysis-and-live-cell-imaging/exosome-labeling-pkh

      도움이 되었습니까?

  4. Will Product PKH67, Green Fluorescent Cell Linker Kit, stain dead cells?

    1 답변

    1. As long as the cell has an intact membrane, the PKH76 dye can label the cell.

      도움이 되었습니까?

  5. What is the difference between Green Fluorescent Cell Linker Kits PKH2 and PKH67?

    1 답변

    1. PKH2 was one of the early PKH dyes.  The PKH67 dye has a longer aliphatic tail.  There is reduced cell-celldye transfer for PKH67 as compared with PKH2.

      도움이 되었습니까?

  6. What method of fixation can be used for tissue/cells with the PKH Fluorescent Cell Linker Kit for General Cell Membrane Labeling dyes?

    1 답변

    1. A protocol for visualization of tissue sections can be found in the technical bulletin.  For visualization of stained cells by immunofluorescence or flow cytometry, the cells can be fixed in 2% paraformaldehyde for 15 minutes. The use of other organic solvents will extract the dye from the cells.  If internal labeling is desired, the cells can be permeabilized with saponin (50-75 μg/mL). Here is the link to the Troubleshooting Guide.

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  7. What is the Department of Transportation shipping information for this product?

    1 답변

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

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  8. What is the excitation and emission spectra for Product PKH67GL, Green Fluorescent Cell Linker Kit?

    1 답변

    1. The spectra can be found on the product insert.The product has a maximum excitation at 490 nm and maximum emission at 502 nm.

      도움이 되었습니까?

  9. How many cells can be stained with Product PKH67GL, Green Fluorescent Cell Linker Kit?

    1 답변

    1. This kit can stain 50 × 107 cells if used as directed (1 × 107 cells stained with 2 × 10-6 M PKH67).

      도움이 되었습니까?

  10. When using Product PKH67GL, PKH67 Green Fluorescent Cell Linker Kit for General Cell Membrane Labeling, how long will the cells remain stained after treatment?

    1 답변

    1. Labeled cells that have been washed can be visualized in culture up to 100 days after staining (for non-dividing cells). The dye itself is stable and will divide equally when the cells divide. After staining with PKH dyes, you can observe as many as 8 divisions depending on how brightly the cells were stained initially and the amount of surface area on the cells. Most commonly, 4-6 divisions can be visualized.

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