The concentration of p-nitrophenylphosphate (pNPP) in this product is 1.5 mg/mL. This item is a ready-to-use product and dilution is not recommended. Please see the link below to review the product datasheet for additional information:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/285/284/p7998dat.pdf
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추천 제품
제품명
Alkaline Phosphatase Yellow (pNPP) Liquid Substrate System for ELISA, ready to use solution
Quality Level
양식
liquid
저장 온도
2-8°C
일반 설명
The product is ready to use buffered alkaline phosphatase substrate consist of p-nitrophenylphosphate (pNPP). This is the advanced substrate system contains insoluble stabilizer that enhances the stability and reactivity as well as reduces the base-line absorption. It produces a soluble end product, hence not recommended for blotting or histochemistry applications.
애플리케이션
Develops a soluble yellow reaction product that may be read at 405 nm. For endpoint assays, the reaction may be stopped with 3 N NaOH. Recommended for ELISA (microwell) procedures; not recommended for membrane applications.
주의사항
Stable at least one year at 2-8 °C.
물리적 형태
Ready to use
제조 메모
Contains p-nitrophenyl phosphate, a substrate for alkaline phosphatase.
신호어
Danger
유해 및 위험 성명서
Hazard Classifications
Eye Dam. 1 - Skin Irrit. 2 - STOT RE 2
Storage Class Code
10 - Combustible liquids
WGK
WGK 3
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
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시험 성적서(COA)
Lot/Batch Number
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문서
NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.
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Hi, may I know what is the concentration of this solution? Because I need to do the calibration curve. Thank you
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도움이 되었습니까?
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Can this be used after it has been frozen?
1 답변-
The stability of this solution has not been evaluated at −20°C. The freeze/thaw process may contribute to decomposition of the substrate solution. Signs of degradation include changes in color or turbidity and precipitation of the pNPP.
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