P7643

PIPES dipotassium salt

≥99% (titration)

• 동의어(들): 1,4-Piperazinediethanesulfonic acid dipotassium salt, Piperazine-1,4-bis(2-ethanesulfonic acid) dipotassium salt
• 실험식(Hill 표기법): C₈H₁₆K₂N₂O₆S₂
• CAS Number: 108321-27-3
• Molecular Weight: 378.55
• MDL number: MFCD00069751
• UNSPSC 코드: 12161700
• PubChem Substance ID: 24898738
• NACRES: NA.25

속성

• 분석: ≥99% (titration)
• 형태: crystalline powder
• 유용한 pH 범위: 6.1-7.5
• pKa(25 °C): 6.8
• solubility: water: 0.25 g/mL, clear, colorless
• 응용 분야: diagnostic assay manufacturing
• SMILES string: [K+].[K+].[O-]S(=O)(=O)CCN1CCN(CC1)CCS([O-])(=O)=O
• InChI: 1S/C8H18N2O6S2.2K/c11-17(12,13)7-5-9-1-2-10(4-3-9)6-8-18(14,15)16;;/h1-8H2,(H,11,12,13)(H,14,15,16);;/q;2*+1/p-2
• InChI key: BJQYFCAMUXGZFN-UHFFFAOYSA-L

설명

일반 설명

PIPES (1,4-Piperazinediethanesulfonic acid) is a zwitterionic buffer. It is a stable buffer and is used majorly in fixation solutions. PIPES is inert towards enzymatic and non-enzymatic reactions. However, it generates artefacts in transmission electron microscope imaging.

애플리케이션

PIPES (1,4-Piperazinediethanesulfonic acid) dipotassium salt has been used as buffer for fluorescence polarization microscopy studies of kinesin-microtubule binding. It is suitable for use as a component of PHEM buffer ([K-PIPES], HEPES, EGTA, and MgSO4) used during rinsing and blocking steps in immunofluorescence imaging of HeLa cells. It is also suitable for use as buffer for purification of tubulin.

안전성 정보

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: Eyeshields, Gloves, type N95 (US)