C3023

CHAPS hydrate

≥98% (HPLC)

• 동의어(들): 3-[(3-Cholamidopropyl)dimethylammonio]-1-propanesulfonate hydrate, CHAPS
• 실험식(Hill 표기법): C₃₂H₅₈N₂O₇S · xH₂O
• CAS Number: 331717-45-4
• Molecular Weight: 614.88 (anhydrous basis)
• MDL number: MFCD00012116
• UNSPSC 코드: 12161900
• PubChem Substance ID: 24892560
• NACRES: NA.21

속성

• 생물학적 소스: bovine bile
• Quality Level: 400
• Grade: HPLC grade
• 설명: zwitterionic
• 분석: ≥98% (HPLC)
• 형태: powder
• 분자량: 614.88 g/mol (anhydrous basis)
• 저장 조건: dry at room temperature (tightly closed)
• 기술: dialysis: suitable; electrophoresis: suitable
• 불순물: <0.04% DMF
• CMC: 8 mM
• mp: 157 °C (dec.) (lit.)
• 전이 온도: cloud point >100 °C
• solubility: water: 50 mg/mL
• 배송 상태: ambient
• 저장 온도: 2-30°C
• SMILES string: O.C[C@H](CCC(=O)NCCC[N+](C)(C)CCCS([O-])(=O)=O)[C@H]1CC[C@H]2[C@@H]3[C@H](O)C[C@@H]4C[C@H](O)CC[C@]4(C)[C@H]3C[C@H](O)[C@]12C
• InChI: 1S/C32H58N2O7S.H2O/c1-21(8-11-29(38)33-14-6-15-34(4,5)16-7-17-42(39,40)41)24-9-10-25-30-26(20-28(37)32(24,25)3)31(2)13-12-23(35)18-22(31)19-27(30)36;/h21-28,30,35-37H,6-20H2,1-5H3,(H-,33,38,39,40,41);1H2/t21-,22+,23-,24-,25+,26+,27-,28+,30+,31+,32-;/m1./s1
• InChI key: SJCUTFKCLFLIFE-JWTJKVBLSA-N

설명

일반 설명

CHAPS hydrate, a zwitterionic detergent derived from Cholic acid, stands out as an excellent tool in biochemical and cell biology research, particularly in the solubilization of membrane proteins. This versatile detergent, combining features of sulfobetaine-type and bile-acid detergents, exhibits a remarkable solubility rate of over 50% in aqueous solutions. It serves a pivotal role in stabilizing protein-DNA complexes, ensuring the retention of biochemical activity in solution.

Widely utilized as a buffer in various research applications, CHAPS hydrate acts as an amphiphilic surfactant, proving effective in solubilizing plasma membrane proteins and diluting assay components. Notably, its easy removal through dialysis or gel filtration adds to its appeal, making it a preferred choice for molecular mass determination by fluorometry. This feature underscores CHAPS hydrate′s adaptability in diverse experimental setups. However, caution is advised in assays involving polyvalent metals or metal ions sensitive to nonspecific chelating agents. Beyond its conventional use, CHAPS hydrate finds application as a polymer matrix in binding assays, effectively removing metal ions from protein solutions.

애플리케이션

0.2 w/v of CHAPS has been used to remove cerebral endothelium by brain perfusion. 2% (w/v) CHAPS has been used as rehydration buffer along with Thiourea and Urea in protein extraction. 10 mM CHAPS has been used along with 0.15 M NaCl, Tris–Cl, pH 7.6, EDTA as lysis buffer in Western Blot analysis. CHAPS has been used as hybridization buffer in in-situ hybridization method of immature mammalian CNS.

특징 및 장점

• Suitable for Biochemical and Cell Biology research
• Can be used in Dialysis and Electrophoresis
• High purity product for research applications

기타 정보

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안전성 정보

• 픽토그램: GHS07
• 신호어: Warning
• 유해 및 위험 성명서: H302
• 예방조치 성명서: P264 - P270 - P301 + P312 - P501
• Hazard Classifications: Acute Tox. 4 Oral
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable