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Merck
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P6611

Millipore

HIS-Select® Nickel Affinity Gel

HIS-Select® Nickel Affinity Gel
1명의 제품 평가자(총 1명)가 샘플 제품을 받았거나 프로모션에 참여했습니다

(1:1 suspension in a 20% ethanol solution)

동의어(들):

Ni-NTA resin, nickel charged agarose

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크기 선택

5 ML
₩280,039
25 ML
₩711,414
100 ML
₩2,650,876
500 ML
₩11,035,588

₩280,039

구입 가능 여부는 고객센터에 문의하십시오.
모든 사진(2)

크기 선택

보기 변경
5 ML
₩280,039
25 ML
₩711,414
100 ML
₩2,650,876
500 ML
₩11,035,588

About This Item

UNSPSC 코드:
12352200
NACRES:
NA.56

₩280,039

구입 가능 여부는 고객센터에 문의하십시오.

결합

magnetic beads

Quality Level

200

양식

(1:1 suspension in a 20% ethanol solution)

특징

hydrophilic

포장

pkg of 1 mL
pkg of 100 mL
pkg of 25 mL
pkg of 5 mL
pkg of 500 mL

농도

1.5-2.4 mL/mL (suspension in packed gel)

기술

protein purification: suitable

색상

faint blue to very dark blue

Matrix

6% Beaded Agarose

용량

>15 mg/mL, gel binding capacity (protein)(with an approx. 30 kDa protein)

전이 온도

flash point 32 °C (closed cup)

저장 온도

2-8°C

관련 카테고리

일반 설명

HIS-Select® Nickel Affinity Gel is an immobilized metal ion affinity chromatography (IMAC) product, used for the purification of His-tagged proteins. While the unique, non-charged, hydrophilic linkage of the proprietary quadridentate NTA chelate group to the beaded agarose charged with nickel ensures high selectivity for small to medium scale His-tag protein purification, it also results in reduced non-specific binding of other proteins. HIS-Select Nickel Affinity Gel is selective for recombinant proteins with His-tags and exhibits low non-specific binding of other proteins. The selectivity can be modulated with the inclusion of imidazole during chromatography.

애플리케이션

HIS-Select® Nickel Affinity Gel has been used in the purification of recombinant proteins like EF-hand calcium-binding protein (S100A14),[1] LIM homeobox transcription factor 1 alpha protein,[2] sigma-1 receptor[3] as well as harpin,[4] stable protein 1 (SP1),[5][6] and BCR-ABL fusion protein.[7]

특징 및 장점

  • High selectivity for higher purity.
  • Unique non-charged hydrophilic linkage reduces non-specific binding.
  • Binding capacity for histidine-tagged protein is greater than 15 mg/mL.
  • Binding under denaturing or non-denaturing conditions.
  • One-step purification.
  • Minimizes unwanted ionic interactions.
  • Minimal nickel leaching.
  • Bead size: 45-165 μm.

결합

It is also available with the EZview™ technology (Product Code E3528).

물리적 형태

1:1 suspension in a 20% ethanol solution

저장 및 안정성

HIS-Select Nickel Affinity Gel is stable for at least one year when stored properly. The HIS-Select Nickel Affinity Gel should be cleaned after each use and an antimicrobial agent such as 20% ethanol should be added to the storage buffer.

법적 정보

HIS-Select is a registered trademark of Merck KGaA, Darmstadt, Germany

관련 제품

제품 번호
설명
가격

08168

Timestrip Plus™ 8 °C

픽토그램

Flame
GHS02

신호어

Warning

유해 및 위험 성명서

H226

Hazard Classifications

Flam. Liq. 3

Storage Class Code

3 - Flammable liquids

WGK

WGK 3

Flash Point (°F)

89.6 °F - closed cup

Flash Point (°C)

32 °C - closed cup

가장 최신 버전 중 하나를 선택하세요:

시험 성적서(COA)

Lot/Batch Number
0000411468
0000411472
0000411470
0000411471
0000411471

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특정 버전이 필요한 경우 로트 번호나 배치 번호로 특정 인증서를 찾을 수 있습니다.

COA 검색

이 제품을 이미 가지고 계십니까?

문서 라이브러리에서 최근에 구매한 제품에 대한 문서를 찾아보세요.

문서 라이브러리 방문

Purification and characterization of the guinea pig sigma-1 receptor functionally expressed in Escherichia coli
Ramachandran S, et al.
Protein Expression and Purification, 51(2), 283-292 (2007)
F Weerkamp et al.
Leukemia, 23(6), 1106-1117 (2009-04-24)
BCR-ABL fusion proteins show increased signaling through their ABL tyrosine kinase domain, which can be blocked by specific inhibitors, thereby providing effective treatment. This makes detection of BCR-ABL aberrations of utmost importance for diagnosis, classification and treatment of leukemia patients.
S100A14, a member of the EF-hand calcium-binding proteins, is overexpressed in breast cancer and acts as a modulator of HER2 signaling
Xu C, et al.
The Journal of Biological Chemistry, 289(2), 827-837 (2014)
Zhijin Zhang et al.
Plant physiology, 150(1), 365-377 (2009-03-06)
Fine-tuning of ethylene production plays an important role in developmental processes and in plant responses to stress, but very little is known about the regulation of ethylene response factor (ERF) proteins in ethylene biosynthesis genes and ethylene production. Identifying cis-acting
Michael B Battles et al.
Nature communications, 8(1), 1528-1528 (2017-11-17)
Human metapneumovirus (hMPV) is a frequent cause of bronchiolitis in young children. Its F glycoprotein mediates virus-cell membrane fusion and is the primary target of neutralizing antibodies. The inability to produce recombinant hMPV F glycoprotein in the metastable pre-fusion conformation
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관련 콘텐츠

Protein Purification

Protein purification techniques, reagents, and protocols for purifying recombinant proteins using methods including, ion-exchange, size-exclusion, and protein affinity chromatography.

단백질 정제

이온교환, 크기 배제 및 단백질 친화성 크로마토그래피를 포함한 방법을 사용하는 재조합형 단백질 정제를 위한 단백질 정제 기법, 시약 및 프로토콜.

단백질 발현

단백질 발현 기술은 연구, 치료제, 백신 생산을 지원하는 다양한 발현 시스템에 사용됩니다.

Protein Expression

Protein expression technologies for various expression systems supporting research, therapeutics, and vaccine production.

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질문

1–6 / 6 질문  

  1. Can Tris buffers be used instead of phosphate buffer for HIS-Select® Nickel Affinity Gel, Product P6611?

    1 답변

    1. Yes, Tris buffers should work.

      도움이 되었습니까?

  2. Can imidazole be used with HIS-Select® Nickel Affinity Gel, Product P6611?

    1 답변

    1. For column chromatography, no more than 20 mM is suggested in the extract, equilibration, and wash buffers to prevent non-specific binding of proteins. No more than 250 mM is suggested for the elution buffers.  Many proteins will elute with imidazole levels as low as 100 to 200 mM.  For batch methods the imidazole concentration may have to be reduced or eliminated.When a protein is expressed at low levels, the presence of the imidazole limits the binding of the protein in the batch method but not when  used in a column.

      도움이 되었습니까?

  3. What is the Department of Transportation shipping information for this product?

    1 답변

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      도움이 되었습니까?

  4. What needs to be done if the HIS-Select® Nickel Affinity Gel, Product P6611, resin turns brown on reuse?

    1 답변

    1. During purification many protein extracts tend to discolor an affinity gel during the loading step. The original color will return after the wash or elution step. If the color is still not changing strip and recharge the affinity gel with nickel.  Wash with EDTA and recharge with Nickel solution.

      도움이 되었습니까?

  5. Can I use SDS with HIS-Select® Nickel Affinity Gel, Product P6611?

    1 답변

    1. 0.1% SDS has been used with some samples, with no adverse effects on the observed protein binding.  However, SDS will effectively coat proteins and may block the binding to the column.  It is probably very  protein-specific and an SDS concentration that works for one protein may not work for another.

      도움이 되었습니까?

  6. Why won't my recombinant protein with a histidine-containing tag bind to HIS-Select® Nickel Affinity Gel, Product P6611?

    1 답변

    1. Verify the pH and composition of sample and equilibration buffers.  Make sure there are no chelating or reducing agents present in the extraction buffer. If using the batch mode, remove imidazole.  Run the affinity purification under denaturing conditions.  Run a Western blot of the extract to verify that the recombinant protein is present.

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