크기 선택
1 ML
₩133,067
10 ML
₩513,387
25 ML
₩893,302
100 ML
₩2,995,468
500 ML
₩12,130,773
₩133,067
구입 가능 여부는 고객센터에 문의하십시오.
모든 사진(1)
크기 선택
보기 변경
1 ML
₩133,067
10 ML
₩513,387
25 ML
₩893,302
100 ML
₩2,995,468
500 ML
₩12,130,773
About This Item
UNSPSC 코드:
41106500
NACRES:
NA.56
₩133,067
구입 가능 여부는 고객센터에 문의하십시오.
추천 제품
양식
suspension
Quality Level
기술
affinity chromatography: suitable
Matrix
Highly cross-linked 6% Beaded Agarose
용량
15 mg/mL, gel binding capacity (protein)(with an approx. 30 kDa protein)
저장 온도
2-8°C
일반 설명
HIS-Select High Flow Nickel Affinity Gel is an immobilized metal-ion affinity chromatography (IMAC) gel that is designed to specifically bind His-tag proteins (histidine containing proteins) in chromatographic systems with pressures up to 200 psi and a maximum linear flow rate of 3,000 cm/hr.
애플리케이션
Affinity gel is suitable for the purification of histidine tagged proteins (His-tag proteins) in native and denaturing conditions.
Nickel Affinity Gel for the purification of HIS TAG® proteins (histidine tagged proteins). HIS-Select® High Flow (HF) brings the superior selectivity of HIS-Select technology to a highly cross-linked agarose for higher flow rates and mechanical stability under pressure. HIS-Select HF is designed for production scale purification and FPLC™ applications. As with other HIS-Select products, the non-charged, hydrophilic linkage of the nickel-nitrilotriacetic acid (Ni-NTA) chelate group to the agarose ensures true one-step purification.
특징 및 장점
- High selectivity for higher purity
- Unique non-charged hydrophilic linkage reduced non-specific binding
- Binding Capacity for histidine-tagged protein (His-tag protein) is greater than 15 mg/mL
- Highly cross-linked agarose matrix allows for purification under higher pressures
- Binding under denaturing or non-denaturing conditions
- One-step purification
물리적 형태
1:1 suspension in a 30% ethanol solution
제조 메모
Ethanol must be removed prior to use. Wash affinity gel in deionized water to remove the ethanol, then equilibrate with equilibration buffer that is provided.
법적 정보
FPLC is a trademark of Cytiva
HIS TAG is a registered trademark of Merck KGaA, Darmstadt, Germany
HIS-Select is a registered trademark of Merck KGaA, Darmstadt, Germany
신호어
Danger
Hazard Classifications
Acute Tox. 4 Oral - Aquatic Chronic 2 - Carc. 1A Inhalation - Flam. Liq. 3 - Muta. 2 - Repr. 1B - Resp. Sens. 1 - Skin Irrit. 2 - Skin Sens. 1 - STOT RE 1 - STOT SE 2
표적 기관
Eyes,Central nervous system, Respiratory Tract
WGK
WGK 3
Flash Point (°F)
88.0 °F
Flash Point (°C)
31.1 °C
개인 보호 장비
Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter
David S Pitcher et al.
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Irving E Vega et al.
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The transition of tau proteins from its soluble physiological conformation to the pathological aggregate forms found in Alzheimer's disease and related dementias, is poorly understood. Therefore, understanding the process that modulates the formation of toxic tau oligomers and their conversion
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Nature chemical biology, 12(12), 1089-1096 (2016-10-25)
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Ivanova, A.S. et al.
Russian journal of developmental biology, 49, 393-397 (2018)
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Journal of medicinal chemistry, 63(16), 8857-8866 (2020-06-12)
DNA-encoded small molecule libraries (DELs) have enabled discovery of novel inhibitors for many distinct protein targets of therapeutic value. We demonstrate a new approach applying machine learning to DEL selection data by identifying active molecules from large libraries of commercial
관련 콘텐츠
Investigate in vitro protein-protein interactions with pull-down assays, utilizing affinity, GST pull-down, TAP, and co-immunoprecipitation methods.
친화성, GST 풀다운, TAP, 공동 면역 침전법을 활용한 풀다운 분석을 통해 in vitro 단백질-단백질 상호작용을 분석합니다.
활성 필터
자사의 과학자팀은 생명 과학, 재료 과학, 화학 합성, 크로마토그래피, 분석 및 기타 많은 영역을 포함한 모든 과학 분야에 경험이 있습니다..
고객지원팀으로 연락바랍니다.
