P3326

Platelet-Derived Growth Factor-AB human

≥97% (SDS-PAGE and N-terminal analysis), recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture

• 동의어(들): PDGF-AB
• MDL number: MFCD00213024
• UNSPSC 코드: 12352202
• NACRES: NA.77

속성

• 제품명: Platelet-Derived Growth Factor-AB human, PDGF-AB, recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture
• 생물학적 소스: human
• Quality Level: 200
• 재조합: expressed in E. coli
• 분석: ≥97% (SDS-PAGE and N-terminal analysis)
• 양식: lyophilized powder
• 효능: 10-100 ng/mL ED₅₀/EC₅₀
• 품질: endotoxin tested
• 분자량: 27-31 kDa
• 포장: pkg of 10 μg; pkg of 50 μg
• 저장 조건: avoid repeated freeze/thaw cycles
• 기술: cell culture | mammalian: suitable
• 불순물: ≤1 EU/μg
• UniProt 수납 번호: P04085, P01127
• 저장 온도: −20°C
• 유전자 정보: human ... PDGFA(5154) , PDGFB(5155)

설명

일반 설명

Platelet-Derived Growth Factor (PDGF) is a cationic polypeptide and a constituent of α-granules. PDGF is composed of chain A and B linked together by a disulphide bridge.[1] PDGF heterodimers occurs as PDGF-AB and homodimers exists as FDGF-AA, PDGF-BB, PDGF-CC and PDGF-DD).[2] The PDGFB gene is localized to pericytes in the brain and is mapped to human chromosome 7p22.3.[3][4]

애플리케이션

Platelet-Derived Growth Factor-AB human has been used in luciferase reporter assay.[5]

생화학적/생리학적 작용

Cellular actions of platelet-derived growth factor (PDGF) include chemoattraction and activation of neutrophils, monocytes, and fibroblasts.[6] PDGF appears to play a crucial role in the cellular response to tissue injury and is critically involved in the pathological progression of atherosclerosis.
PDGF stimulates fibroblast proliferation and extracellular matrix production in order to facilitate tissue repair.[7] PDGF-AB isomer is found to be associated with angiogenesis and proliferation.[8] Loss of PDGFB gene function induces microvascular calcification due to age-dependent phenotypical changes.[3]

기타 정보

Heterodimer of the A and B chains of PDGF disulfide linked.

물리적 형태

Lyophilized from a 0.2 μm filtered solution in 40% acetonitrile and 0.1% trifluoroacetic acid.

분석 메모

The biological activity is measured by its ability to stimulate ³H-thymidine incorporation in quiescent NR6R-3T3 fibroblasts.

안전성 정보

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: Eyeshields, Gloves, type N95 (US)