콘텐츠로 건너뛰기
Merck
모든 사진(2)

주요 문서

모든 문서 보기

P2850

Sigma-Aldrich

Phosphatase Inhibitor Cocktail 1

DMSO solution, mixture of inhibitors

동의어(들):

Phosphatase Inhibitor

로그인조직 및 계약 가격 보기

크기 선택

1 ML
₩146,972
5 ML
₩675,381

₩146,972

구입 가능 여부는 고객센터에 문의하십시오.
벌크 견적 요청
모든 사진(2)

크기 선택

보기 변경
1 ML
₩146,972
5 ML
₩675,381

About This Item

EC Number:
200-664-3
UNSPSC 코드:
12352200
NACRES:
NA.54

₩146,972

구입 가능 여부는 고객센터에 문의하십시오.
벌크 견적 요청

제품명

Phosphatase Inhibitor Cocktail 1, DMSO solution

양식

DMSO solution

Quality Level

300

배송 상태

wet ice

저장 온도

2-8°C

일반 설명

Phosphatase Inhibitor Cocktail 1 includes a mixture of inhibitors that will inhibit the L-isozymes of alkaline phosphatase as well as serine/threonine protein phosphatases such as PP1 and PP2A. This product contains microcystinLR, cantharidin, and (−)-p-bromotetramisole. Phosphatase Inhibitor Cocktail 1 has been replaced with P0044.

애플리케이션

Phosphatase Inhibitor Cocktail 1 has been used in nuclear protein isolation and electrophoretic mobility shift assay (EMSA) for catenin activation.[1] It has also been used in western blot analysis.[2][3]

생화학적/생리학적 작용

Phosphatases are active regulators of a number of important biological processes. Inhibition of phosphatases by small molecules is useful to study its biological role.[4]

수량

Recommended usage is a 100-fold dilution.

분석 메모

Tested on cell extracts from animal tissues such as bovine liver, human placenta, and rabbit muscle, as well as on extracts from A431 and Jurkat cell lines.

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point (°F)

188.6 °F

Flash Point (°C)

87 °C

가장 최신 버전 중 하나를 선택하세요:

시험 성적서(COA)

Lot/Batch Number
0000317618
0000317617
0000215517
056M4081V
17160104

적합한 버전을 찾을 수 없으신가요?

특정 버전이 필요한 경우 로트 번호나 배치 번호로 특정 인증서를 찾을 수 있습니다.

COA 검색

이 제품을 이미 가지고 계십니까?

문서 라이브러리에서 최근에 구매한 제품에 대한 문서를 찾아보세요.

문서 라이브러리 방문

Class III β-tubulin expression and in vitro resistance to microtubule targeting agents.
Stengel C, et al.
British Journal of Cancer, 102(2), 316-316 (2010)
Larger therapeutic window for steroid versus VEGF-A inhibitor in inflammatory angiogenesis: surprisingly similar impact on leukocyte infiltration.
Nakao S, et al.
Investigative Ophthalmology & Visual Science, 53(7), 3296-3302 (2012)
Discovery of protein phosphatase inhibitor classes by biology-oriented synthesis.
Noren M A, et al.
Proceedings of the National Academy of Sciences of the USA, 103(28), 10606-10611 (2006)
X Zhao et al.
Mucosal immunology, 10(6), 1553-1568 (2017-04-13)
Virulent Mycobacterium tuberculosis (Mtb) triggers necrosis in host Mϕ, which is essential for successful pathogenesis in tuberculosis. Here we demonstrate that necrosis of Mtb-infected Mϕ is dependent on the action of the cytosolic Receptor Interacting Protein Kinase 3 (RIPK3) and
Aberrant Wnt/β-catenin signaling in pancreatic adenocarcinoma.
Zeng G, et al.
Neoplasia, 8(4), 279-289 (2006)
모든 관련된 서류 보기

문서

Phosphatase Inhibitor Cocktail

The use of phosphatase inhibitors is critical for all types of phosphorylation studies. Compare phosphatase inhibitor cocktails to select the best one for your research.

질문

1–6 / 6 질문  

  1. What is the Department of Transportation shipping information for this product?

    1 답변

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      도움이 되었습니까?

  2. How stable is Product P2850, Phosphatase Inhibitor Cocktail 1?  Can I freeze it?

    1 답변

    1. This reagent, as supplied and stored at 2-8°C, will be stable for four years from the date of manufacturing. Freezing is not recommended, because it may cause precipitation of the inhibitors.

      도움이 되었습니까?

  3. Product P2850, Phosphatase Inhibitor Cocktail 1, is solid when it arrived. Is it suitable to use?

    1 답변

    1. Since this solution is supplied in DMSO, it may solidify (melting point of DMSO is 16-19 deg C).  Simply allow it to melt before use.  This will not affect the product.

      도움이 되었습니까?

  4. What inhibitors are present in Product P2850, Phosphatase Inhibitor Cocktail 1, and in what concentrations?

    1 답변

    1. Cantharidin (Product Code C7632) inhibits protein phosphatase 2A (PP-2A).  Bromotetramisole (Product Code B8132) inhibits alkaline phosphatases. Microcystin LR (Product Code M2912) inhibits protein phosphatases 1 and 2A (PP-1 and PP-2A). The concentrations of these inhibitors are considered proprietary.

      도움이 되었습니까?

  5. At what concentration should I use Product P2850, Phosphatase Inhibitor Cocktail 1?

    1 답변

    1. In most circumstannces, the product is used at a 100-fold dilution.  This will inhibit the phosphatases in 5 mg of protein per mL of lysate.

      도움이 되었습니까?

  6. Is Product P2850, Phosphatase Inhibitor Cocktail 1, suitable for use on yeast?

    1 답변

    1. As far as we know, the phosphatases in mammalian cells also appear in yeast cells. Thus both cocktails, P5726 (Phosphatase cocktail II and P2850 (Phosphatase Inhibitor cocktail I), may be needed with yeast extracts.

      도움이 되었습니까?

후기

평점 값 없음

활성 필터

자사의 과학자팀은 생명 과학, 재료 과학, 화학 합성, 크로마토그래피, 분석 및 기타 많은 영역을 포함한 모든 과학 분야에 경험이 있습니다..

고객지원팀으로 연락바랍니다.