MSSAFE

Protease and Phosphatase Inhibitor Cocktail

MS-SAFE, powder, Protease inhibitors: serine, cysteine and aspartic proteases and metalloproteases. Phosphatase inhibitors: tyrosine, serine/threonine, acid and alkaline phosphatases.

• 동의어(들): Mass Spectrometry Safe Protease and Phosphatase Inhibitor Cocktail
• UNSPSC 코드: 12352200
• NACRES: NA.77

속성

• product name: MS-SAFE Protease and Phosphatase Inhibitor,
• 설명: for use with mammalian cell and tissue extract, lyophilized powder
• Quality Level: 200
• 형태: powder
• solubility: water: 0.1 g/mL, clear, colorless
• 저장 온도: 2-8°C

설명

일반 설명

Mass spectrometry (MS) compatible protease inhibitor cocktail and phosphatase inhibitor cocktail, with broad specificity for the inhibition of:

• Serine, cysteine and aspartic proteases, and metalloproteases
• Tyrosine, serine/threonine, acid and alkaline phosphatases

All inhibitors and fillers were chosen so as not to interfere with LC-MS analyses. Any inhibitors capable of covalent, irreversible protein modification were avoided. This cocktail is EDTA-free. All inhibitors were selected to allow facile downstream sample processing, such as immobilized metal affinity chromatography (IMAC) for His-tagged protein purification and, uniquely, phosphopeptide enrichment.

특이성

Protease inhibitors: serine, cysteine and aspartic proteases, and metalloproteases
Phosphatase inhibitors: tyrosine, serine/threonine, acid and alkaline phosphatases

애플리케이션

MS-SAFE Protease and Phosphatase Inhibitor has been used to measure ribosomal protein S6 kinase (S6K) activity.

Tested in mammalian cell lysates and liver tissue extracts. Designed for use in samples to be analyzed by mass spectrometry.

특징 및 장점

Comprehensive protease and phosphatase inhibitor cocktail for mass spectrometry analysis

Compatible with downstream sample processing such as His-tagged protein purification and phosphopeptide enrichment

Allows accurate measurement of protein activity and identification of phosphorylation sites.

성분

Protease inhibitors:

• Bestatin hydrochloride
• Leupeptin
• Phosphoramidon disodium salt
• Pepstatin A
• Elastatinal
• Aprotinin
• Nafamostat mesylate
• Antipain

Phosphatase inhibitors

• Okadaic acid
• Sodium fluoride
• Sodium orthovanadate
• Bromotetramisole oxalate

Fillers

• β-lactose
• DL-leucine

수량

One vial makes 20 mL of 1× inhibitor cocktail working solution, using either water or extraction/lysis buffer. Alternatively, a 10× concentrated solution may be prepared by adding 2 mL of water or extraction/lysis buffer. This 10× solution may then be diluted 10-fold into extraction/lysis buffer as needed for a 1× working solution.

물리적 형태

Lyophilized powder that is water-soluble

안전성 정보

• 픽토그램: GHS07
• 신호어: Warning
• 유해 및 위험 성명서: H302,H315,H319
• 예방조치 성명서: P264 - P270 - P280 - P301 + P312 - P302 + P352 - P305 + P351 + P338
• Hazard Classifications: Acute Tox. 4 Oral - Eye Irrit. 2 - Skin Irrit. 2
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable