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To collect the agarose beads, a centrifugation speed of 1000 x g for 5 minutes should be sufficient, using the A2220 batch method as a guide
P0762
Phosphatase, Alkaline−Agarose from calf intestine
ammonium sulfate suspension
동의어(들):
Alkaline phosphatase-Agarose
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50 UNITS
₩420,921
100 UNITS
₩739,022
250 UNITS
₩1,601,772
1000 UNITS
₩5,178,936
₩420,921
출고 가능일2025년 3월 28일세부사항
모든 사진(1)
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보기 변경
50 UNITS
₩420,921
100 UNITS
₩739,022
250 UNITS
₩1,601,772
1000 UNITS
₩5,178,936
About This Item
₩420,921
출고 가능일2025년 3월 28일세부사항
추천 제품
생물학적 소스
bovine (calf) intestine
양식
ammonium sulfate suspension
특이 활성도
250-1000 units/mL packed gel
Matrix
beaded agarose
저장 온도
2-8°C
일반 설명
Alkaline phosphatase from calf intestine is a glycoprotein with a molecular weight of 140,000. [1]
애플리케이션
Alkaline phosphatase from calf intestine, CIP, removes phosphate groups from the 5′ ends of DNA which aids in cloning techniques to keep DNA from self-ligation. It can be inhibited by homoarginine. [2]
Calf intestinal alkaline phosphatase (CIAP) has been used in a study with the spectrophotometric method to assess the inhibition effect of dansyl-L-phenylalanine. [3] It has also been shown that (CIAP) is able to detoxify the lipopolysaccharide (LPS)-mediated inflammatory response, which could be important in the treatment of LPS-mediated diseases. [4]
생화학적/생리학적 작용
Sigma insoluble enzymes are produced by reacting the conventional "soluble" enzyme with an inert base that results in an insoluble compound retaining the activity of the original enzyme. Applications are almost unlimited. By selecting the "inert" base carefully, a highly active "resin" or "gel" can be produced with which the enzyme reaction can be quickly catalyzed by momentary contact with the substrate in a suitable medium. This can be carried out either in a batch slurry or even through a small column. In either case, the insoluble enzyme does not remain as a contaminant of the reaction mix. It can be readily filtered or centrifuged out of the batch slurry, or remain behind in the column. Many unstable enzymes seem to be much more stable in the insoluble form.
포장
Package size based on DEA units
단위 정의
One unit will hydrolyze 1.0 mmole of p-nitrophenyl phosphate per min at pH9.8 at 37 °C.
물리적 형태
Suspension in 2.0 M (NH4)2SO4, 1 mM MgCl2, 0.1 mM ZnCl2, pH 7
Storage Class Code
10 - Combustible liquids
WGK
WGK 3
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
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시험 성적서(COA)
Lot/Batch Number
Rebika Shrestha et al.
iScience, 25(1), 103608-103608 (2022-02-03)
Understanding the spatiotemporal distribution and dynamics of RAS on the plasma membrane (PM) is the key for elucidating the molecular mechanisms of the RAS signaling pathway. Single particle tracking (SPT) experiments show that in cells, KRAS diffuses in at least
Li-Na Li et al.
Guang pu xue yu guang pu fen xi = Guang pu, 29(10), 2820-2823 (2009-12-30)
To evaluate the inhibition effect of dansyl-L-phenylalanine on calf intestinal alkaline phosphatase (CIAP), UV-Vis spectrophotometric method was employed. It was found that dansyl-L-phenylalanine can selectively inhibit CIAP. The kinetic inhibition processes of dansyl-L-phenylalanine and L-phenylalanine were comparatively studied. The authors'
J E Coleman
Annual review of biophysics and biomolecular structure, 21, 441-483 (1992-01-01)
Alkaline phosphatase was the first zinc enzyme to be discovered in which three closely spaced metal ions (two Zn ions and one Mg ion) are present at the active center. Zn ions at all three sites also produce a maximally
M Kellerer et al.
Diabetologia, 41(7), 833-838 (1998-08-01)
Protein kinase C (PKC) isoforms are potentially important as modulators of the insulin signalling chain and could be involved in the pathogenesis of cellular insulin resistance. We have previously shown that phorbol ester stimulated PKC beta1 and beta2 as well
Calf intestinal alkaline phosphatase, a novel therapeutic drug for lipopolysaccharide (LPS)-mediated diseases, attenuates LPS toxicity in mice and piglets
Beumer, C.
Journal of Pharmacology and Experimental Therapeutics, 397, 737-744 (2003)
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