Yes, this kit has been referenced to work with induced pluripotent stem cells (iPSCs). Although not tested, using a thermocycler set to 2-4°C will effectively maintain the required conditions as a viable alternative to using ice for the incubation steps during the nuclei isolation process. Please see the link below to review the publication referencing use with iPSCs.
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6992778/
₩610,523
재고 있음세부사항
추천 제품
사용
sufficient for 25 nuclei preparations (~1-10×107 cells/preparation)
Quality Level
유통기한
1 yr at 2‑8 °C
포장
pkg of 1 kit
응용 분야
cell analysis
외래 활성
nuclease and protease, free
배송 상태
wet ice
저장 온도
2-8°C
일반 설명
애플리케이션
생화학적/생리학적 작용
기타 정보
관련 제품
Storage Class Code
10 - Combustible liquids
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
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시험 성적서(COA)
이미 열람한 고객
문서
Centrifugation separates organelles based on size, shape, and density, facilitating subcellular fractionation across various samples.
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Can this buffer be used with ipscs and can the incubation be conducted in a thermocyler at 2-4C instead of on ice
1 답변-
도움이 되었습니까?
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What is the pH of the EZ-prep lysis buffer
1 답변-
The complete formulation for the Lysis solution is given in the following reference. The lysis buffer in the kit is formulated the same, except without the Triton X-100 and the DTT.
“Analysis of nuclear RNA”, Chapter 19, in RNA Methodologies: A Laboratory Guide for Isolation and Characterization, Robert E. Farrell, Jr., Academic Press, San Diego, p. 406-437 (1998)도움이 되었습니까?
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Is the Nuclei isolation Kit Nuclei EZ Prep designed for DNA or protein isolation?
1 답변-
The Nuclei isolation Kit Nuclei EZ Prep is designed for the isolation of intact nuclei containing nucleic acid and protein factors.
도움이 되었습니까?
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What is the formulation of the storage solution of NUC101?
1 답변-
The storage solution for NUC101 (Product No. S8933) contains 2 mM MgCl2, 0.1 mM Na2EDTA, and no detergent in a Tris buffer (pH 8.0) with 30% glycerol.
도움이 되었습니까?
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Can this kit be used with vertebrate cells which are not mammalian (such a avian cells)?
1 답변-
Product NUC101 has been validated for use with mammalian samples. However, it has been used in many different species by independent laboratories. One study used Nuc201 with zebrafinch samples:
https://www.nature.com/articles/s41467-021-22918-2도움이 되었습니까?
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For the upcoming important phase of our project, we need the Nuclei EZ Prep Nuclei Isolation Kit. Do you have alternatives to this product in your range, which we can use for our Nuclei Isolation? Or are there any remaining stocks that we can use.
1 답변-
The NUC201 Nuclei Isolation Kit is an available alternative for this product. The NUC201 method includes centrifugation in a sucrose solution which offers additional protection to the nuclei, but may result in a lower yield when compared to the NUC101. Please see the link below to review the product datasheet:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/210/075/nuc201bul.pdfFor additional questions regarding this product option, we kindly ask you to navigate to the link https://www.sigmaaldrich.com/techservice, click on "Product Technical Inquires" under the Products Section with all the required information so that a member of our team can reach out to you to assist further. Thank you.
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What is the Department of Transportation shipping information for this product?
1 답변-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
도움이 되었습니까?
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Should I use trypsin to harvest my adherent cells before using Product NUC101, Nuclei Isolation Kit?
1 답변-
In general, this is not advised. We have never tried detaching the cells first with trypsin. We would not recommend it, because residual trypsin could clip nuclear proteins and cause problems in downstream applications.
도움이 되었습니까?
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Can Product NUC101, Nuclei Isolation Kit, be used to capture soluble nuclear proteins?
1 답변-
The kit was originally designed to produce nuclei that are competent for transcription run-on/run-off assays to measure transcription from pre-engaged polymerase complexes on the nuclear DNA. It was not designed for the separation or isolation of soluble nuclear proteins. The researcher might be advised to try the NXTRACT nuclear extraction kit, which allows for the isolation of "intact" nuclei from other cellular components before the lysis of the nuclear membrane.
도움이 되었습니까?
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Can I use Product NUC101, Nuclei Isolation Kit, on frozen cells?
1 답변-
It is likely that the freezing and thawing processes will damage many of the cells and nuclei, which could lead to loss of nuclear components and/or contamination of the nuclei with cytoplasmic or other cellular components.
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