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Merck
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주요 문서

NUC101

Sigma-Aldrich

Nuclei Isolation Kit: Nuclei EZ Prep

sufficient for 25 nuclei preparations (~1-10×107 cells/preparation)

동의어(들):

Rapid mammalian nuclei isolation kit

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크기 선택

1 KIT
₩610,523

₩610,523


재고 있음세부사항



크기 선택

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1 KIT
₩610,523

About This Item

UNSPSC 코드:
12352207
NACRES:
NA.32

₩610,523


재고 있음세부사항


사용

sufficient for 25 nuclei preparations (~1-10×107 cells/preparation)

Quality Level

유통기한

1 yr at 2‑8 °C

포장

pkg of 1 kit

응용 분야

cell analysis

외래 활성

nuclease and protease, free

배송 상태

wet ice

저장 온도

2-8°C

일반 설명

The Nuclei EZ Prep Kit was developed for the rapid isolation of nuclei from mammalian cells. The kit provides a high yield of nuclei from commonly used cell types.

애플리케이션

Suitable as a source of nuclear components, to produce nuclei for in vitro apoptosis assays, and for functional studies.

생화학적/생리학적 작용

The protocol provides a high yield of nuclei from commonly used mammalian cells, including both adherent (e.g., HEK293 and COS7) and non-adherent (e.g., Jurkat and HFN7.1) cell lines and peripheral blood mononuclear cells (PBMCs). The preparations are suitable for many cell biology applications, e.g., as a source of nuclear components such as chromatin, genomic DNA, histones and nuclear RNA/RNP, produces nuclei for in vitro apoptosis assays, and functional studies such as examination of the transcriptional status of cells.

기타 정보

All procedures should be carried out on ice or 2-8°C.

관련 제품

제품 번호
설명
가격

Storage Class Code

10 - Combustible liquids

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable


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시험 성적서(COA)

Lot/Batch Number

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특정 버전이 필요한 경우 로트 번호나 배치 번호로 특정 인증서를 찾을 수 있습니다.

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문서 라이브러리에서 최근에 구매한 제품에 대한 문서를 찾아보세요.

문서 라이브러리 방문

Christina N Vallianatos et al.
Communications biology, 3(1), 278-278 (2020-06-03)
Histone H3 lysine 4 methylation (H3K4me) is extensively regulated by numerous writer and eraser enzymes in mammals. Nine H3K4me enzymes are associated with neurodevelopmental disorders to date, indicating their important roles in the brain. However, interplay among H3K4me enzymes during
Analysis of nuclear RNA, Chapter 14.
Robert E. Farrell, Jr., ed.
RNA Methodologies: A Laboratory Guide for Isolation and Characterization, 235-263 (1993)
Buqing Ye et al.
Nature communications, 8(1), 1518-1518 (2017-11-16)
Lymphoid lineage commitment is an important process in haematopoiesis, which forms the immune system to protect the host from pathogen invasion. However, how multipotent progenitors (MPP) switch into common lymphoid progenitors (CLP) or common myeloid progenitors (CMP) during this process
Yu-Yin Shih et al.
PloS one, 6(10), e26236-e26236 (2011-10-25)
Retinoic acid (RA) has been approved for the differentiation therapy of neuroblastoma (NB). Previous work revealed a correlation between glucose-regulated protein 75 (GRP75) and the RA-elicited neuronal differentiation of NB cells. The present study further demonstrated that GRP75 translocates into
Srikanth Pendyala et al.
Antioxidants & redox signaling, 11(4), 747-764 (2008-09-12)
In vascular endothelium, the major research focus has been on reactive oxygen species (ROS) derived from Nox2. The role of Nox4 in endothelial signal transduction, ROS production, and cytoskeletal reorganization is not well defined. In this study, we show that

문서

Centrifugation separates organelles based on size, shape, and density, facilitating subcellular fractionation across various samples.

질문

1–10 / 10 질문  
  1. Can this buffer be used with ipscs and can the incubation be conducted in a thermocyler at 2-4C instead of on ice 

    1 답변
    1. Yes, this kit has been referenced to work with induced pluripotent stem cells (iPSCs). Although not tested, using a thermocycler set to 2-4°C will effectively maintain the required conditions as a viable alternative to using ice for the incubation steps during the nuclei isolation process. Please see the link below to review the publication referencing use with iPSCs.
      https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6992778/

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  2. What is the pH of the EZ-prep lysis buffer

    1 답변
    1. The complete formulation for the Lysis solution is given in the following reference. The lysis buffer in the kit is formulated the same, except without the Triton X-100 and the DTT.
      “Analysis of nuclear RNA”, Chapter 19, in RNA Methodologies: A Laboratory Guide for Isolation and Characterization, Robert E. Farrell, Jr., Academic Press, San Diego, p. 406-437 (1998)

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  3. Is the Nuclei isolation Kit Nuclei EZ Prep designed for DNA or protein isolation?

    1 답변
    1. The Nuclei isolation Kit Nuclei EZ Prep is designed for the isolation of intact nuclei containing nucleic acid and protein factors.

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  4. What is the formulation of the storage solution of NUC101?

    1 답변
    1. The storage solution for NUC101 (Product No. S8933) contains 2 mM MgCl2, 0.1 mM Na2EDTA, and no detergent in a Tris buffer (pH 8.0) with 30% glycerol.

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  5. Can this kit be used with vertebrate cells which are not mammalian (such a avian cells)?

    1 답변
    1. Product NUC101 has been validated for use with mammalian samples. However, it has been used in many different species by independent laboratories. One study used Nuc201 with zebrafinch samples:
      https://www.nature.com/articles/s41467-021-22918-2

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  6. For the upcoming important phase of our project, we need the Nuclei EZ Prep Nuclei Isolation Kit. Do you have alternatives to this product in your range, which we can use for our Nuclei Isolation? Or are there any remaining stocks that we can use.

    1 답변
    1. The NUC201 Nuclei Isolation Kit is an available alternative for this product. The NUC201 method includes centrifugation in a sucrose solution which offers additional protection to the nuclei, but may result in a lower yield when compared to the NUC101. Please see the link below to review the product datasheet:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/210/075/nuc201bul.pdf

      For additional questions regarding this product option, we kindly ask you to navigate to the link https://www.sigmaaldrich.com/techservice, click on "Product Technical Inquires" under the Products Section with all the required information so that a member of our team can reach out to you to assist further. Thank you.

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  7. What is the Department of Transportation shipping information for this product?

    1 답변
    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

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  8. Should I use trypsin to harvest my adherent cells before using Product NUC101, Nuclei Isolation Kit?

    1 답변
    1. In general, this is not advised. We have never tried detaching the cells first with trypsin. We would not recommend it, because residual trypsin could clip nuclear proteins and cause problems in downstream applications.

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  9. Can Product NUC101, Nuclei Isolation Kit, be used to capture soluble nuclear proteins?

    1 답변
    1. The kit was originally designed to produce nuclei that are competent for transcription run-on/run-off assays to measure transcription from pre-engaged polymerase complexes on the nuclear DNA. It was not designed for the separation or isolation of soluble nuclear proteins. The researcher might be advised to try the NXTRACT nuclear extraction kit, which allows for the isolation of "intact" nuclei from other cellular components before the lysis of the nuclear membrane.

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  10. Can I use Product NUC101, Nuclei Isolation Kit, on frozen cells?

    1 답변
    1. It is likely that the freezing and thawing processes will damage many of the cells and nuclei, which could lead to loss of nuclear components and/or contamination of the nuclei with cytoplasmic or other cellular components.

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