Quality Segment
usage
sufficient for 50 g (tissue)
technique(s)
fractionation: suitable
input
mammalian sample(s)
shipped in
wet ice
storage temp.
2-8°C
General description
The Golgi Isolation Kit provides a method for isolating Golgi membranes from mammalian soft tissues by discontinuous density gradient. The degree of Golgi enrichment can be determined by assaying the acitivty of UDP-galactosyl transferase or by immunodetection of Golgi specific marker proteins like B-COP or GM130 using appropriate antibodies (Cat. No. G6160 and G7295, respectively). Separation from other organelles can be measured using the appropriate marker detection kits (Cat. No. CS0780, CYTOCOX1, CY0100 and CAT100).
Application
Golgi Isolation Kit may be used for the isolation of Golgi membranes from mammalian soft tissues by discontinuous density gradient.
Analysis Note
The Golgi Isolation kit was optimized using rat liver and tested on rat kidney, spleen, and heart.
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이 품목 | |||
|---|---|---|---|
| description sufficient for 50 g (tissue) | description isolate peroxisomes from tissues and cells | description sufficient for 10-20 g (animal tissue), sufficient for 50 assays (2 mL), isolation of enriched mitochondrial fraction from animal tissues | description BioUltra, Molecular Biology |
| technique(s) fractionation: suitable | technique(s) centrifugation: suitable, fractionation: suitable | technique(s) fractionation: suitable | technique(s) - |
| usage sufficient for 50 g (tissue) | usage - | usage sufficient for 10-20 g (animal tissue), sufficient for 50 assays (2 mL) | usage - |
| input mammalian sample(s) | input mammalian sample(s) | input mammalian sample(s) | input - |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 100 |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. −20°C | storage temp. 2-8°C |
| shipped in wet ice | shipped in wet ice | shipped in dry ice | shipped in - |
키트 구성품 역시 별도로 이용 가능함
제품 번호
설명
SDS & 가격
- Protease Inhibitor Cocktail, for use with mammalian cell and tissue extracts, DMSO solution 5 mL
저장 등급
10 - Combustible liquids
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