N2513

Nerve Growth Factor β rat

≥97% (SDS-PAGE), recombinant, expressed in Sf21 cells, lyophilized powder, suitable for cell culture

• 동의어(들): NGF-β from rat, Nerve Growth Factor-β from rat
• CAS Number: 86923-98-0
• MDL number: MFCD00164982
• UNSPSC 코드: 12352202
• NACRES: NA.77

속성

• 제품명: NGF-β from rat, recombinant, expressed in Sf21 cells, lyophilized powder, suitable for cell culture
• 생물학적 소스: rat
• Quality Level: 200
• 재조합: expressed in Sf21 cells
• 분석: ≥97% (SDS-PAGE)
• 양식: lyophilized powder
• 효능: 0.15-3.00 ng/mL EC₅₀
• 포장: pkg of 0.1 mg
• 기술: cell culture | mammalian: suitable
• 불순물: endotoxin, tested
• solubility: water: soluble 0.1 g/L at 20 °C
• UniProt 수납 번호: P25427
• 저장 온도: −20°C
• 유전자 정보: rat ... NGF(4803)

설명

일반 설명

NGFβ (nerve growth factor β) is a protein involved in signal transduction, and expressed by multiple tissues such as placenta, urothelium and bladder smooth muscle cells.[1][2] It is a neurotrophin and belongs to the NGF family.[3]

애플리케이션

NGF-β from rat is used for the following applications:

• In the preparation of DMEM medium (for the cultivation and differentiation of PC12 cells)[4][5]
• Western blotting analysis[6]
• For the preparation of differentiation medium (it is prepared from DMEM, in which NGF-β is one of the components)[7]
• In cell culture and treatment (for neuronal differentiation)[8]
• In the preparation of stock solution[9]

생화학적/생리학적 작용

NGFβ (nerve growth factor β) is essential for the differentiation and survival of neurons. It also plays a key role in the development and maintenance of nervous system. It is also involved in angiogenesis, and facilitates tumorigenesis such as, oral, breast and prostate cancers.[3] It has potential as marker for the diagnosis of interstitial cystitis/painful bladder syndrome (IC/PBS) and overactive bladder (OAB), and might also aid in determining the therapy for the same.[1] It might be involved in changes in placental function, as NGF signaling is influenced by antenatal depression and selective serotonin reuptake inhibitor (SSRI) treatment.[2]

물리적 형태

Lyophilized from a 0.2 μm filtered solution in PBS and 1 M NaCl containing 50 μg of bovine serum albumin per 1 μg of cytokine

분석 메모

The biological activity is measured in a cell proliferation assay using the factor-dependent human erythroleukemic cell line, TF-1.

안전성 정보

• Storage Class Code: 13 - Non Combustible Solids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: Eyeshields, Gloves, type N95 (US)