The supplier has not tested MAK459 with tissue samples, but it is possible that it would work. It's important to note that samples containing bile acids such as sodium deoxycholate can interfere with this assay and should be avoided. Additionally, samples should be clear and free of particles or turbidities.
추천 제품
Quality Level
저장 조건
(No metal containers. Tightly closed.)
입력
protein
water
soil
응용 분야
agriculture
environmental
검출 방법
colorimetric
저장 온도
2-8°C
일반 설명
Detergents play a significant role in increasing environmental pollution across various environments. This pollution originates from wastewater sources, such as residential areas where household detergents are used, agricultural runoff containing herbicides and insecticides, and certain industries.In biochemical research, detergents are commonly used for membrane protein separation. However, they can interfere with protein assays by affecting protein-protein interactions, enzymatic activities, or protein structure. Detecting and quantifying the presence of detergents is essential for accurate measurements in protein assays. Detergents used during protein extraction or purification processes may carry over into downstream applications, such as electrophoresis, chromatography, or mass spectrometry. This is crucial for maintaining assay accuracy, assessing protein functionality, ensuring compatibility with downstream applications, and preserving sample integrity.
애플리케이션
The Detergent Assay Kit may be used for:
- Soil Testing
- Water Testing
- Detergent Detection in Biological Samples
특징 및 장점
Efficient Procedure: Streamline your workflow with a hassle-free 15-minute process, requiring only the addition of a single working reagent and a small 20 μL sample size, saving you valuable time and effort.
Linear Detection Range:
Linear Detection Range:
- Tween 80: Detect concentrations ranging from 0.012 to 4 mM
- Tween 20: Detect concentrations ranging from 0.06 to 5 mM
- Triton X-100: Detect concentrations ranging from 0.23 to 12 mM
- Brij L23/35: Detect concentrations ranging from 0.09 to 5 mM
- DTAC: Detect concentrations ranging from 0.08 to 2 mM
- SDS: Detect concentrations ranging from 10 to 25 mM
적합성
The kit is suitable for detecting traces of detergent in purified protein samples and the determination of detergent in water and soil samples.
원리
Simple, direct and automation-ready procedures for measuring detergent concentration in biological samples are very desirable. The Detergent Assay Kit is designed to measure detergent directly without any pretreatment. Above the critical micellar concentration, detergent forms micelle in solution that trap the colorimetric dye. The intensity of the color is directly proportional to the detergent concentration in the sample.
기타 정보
For additional information on our range of Biochemicals, please complete this form.
신호어
Danger
유해 및 위험 성명서
Hazard Classifications
Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Met. Corr. 1
Storage Class Code
8B - Non-combustible corrosive hazardous materials
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
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시험 성적서(COA)
Lot/Batch Number
활성 필터
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