추천 제품
검출 방법
fluorometric
관련 질환(들)
hematological disorder
저장 온도
−20°C
일반 설명
Thrombin enzyme (Factor IIa, EC 3.4.21.5), a Serine protease, is an important clotting factor in the coagulation cascade that involves the conversion of soluble fibrinogen to insoluble active fibrin strands. In this pathway, Prothrombin is proteolytically converted into an active Thrombin.
적합성
Suitable for determining the activity of pure Thrombin and for detecting the activity of Thrombin in plasma.
원리
Thrombin activity fluorometric assay kit utilizes the ability of thrombin to proteolytically cleave a synthetic substrate and release a fluorophore, 7-amino-4-methylcoumarin (AMC), which can be easily quantified by fluorescence. This assay kit is simple, rapid and can detect thrombin activity as low as 1ng in samples.
Storage Class Code
10 - Combustible liquids
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시험 성적서(COA)
Lot/Batch Number
이미 열람한 고객
The conversion of prothrombin to thrombin I. Characterization of the reaction products formed during the activation of bovine prothrombin.
Owen W G, et al.
The Journal of Biological Chemistry, 249(2), 594-605 (1974)
Crystal structure of the thrombin-hirudin complex: a novel mode of serine protease inhibition.
Grutter M G, et al.
The Embo Journal, 9(8), 2361-2365 (1990)
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