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Merck
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주요 문서

L0144

Sigma-Aldrich

Lucifer Yellow CH dipotassium salt

powder

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크기 선택

25 MG
₩252,634
100 MG
₩761,352

₩252,634


출고 가능일2025년 4월 10일세부사항


벌크 견적 요청

크기 선택

보기 변경
25 MG
₩252,634
100 MG
₩761,352

About This Item

실험식(Hill 표기법):
C13H9K2N5O9S2
CAS Number:
Molecular Weight:
521.57
Beilstein:
5224942
MDL number:
UNSPSC 코드:
12171500
PubChem Substance ID:
NACRES:
NA.77

₩252,634


출고 가능일2025년 4월 10일세부사항


벌크 견적 요청

양식

powder

색상

orange

형광

λex 428 nm; λem 540 nm

저장 온도

room temp

SMILES string

[K+].[K+].NNC(=O)NN1C(=O)c2cc(cc3c(N)c(cc(C1=O)c23)S([O-])(=O)=O)S([O-])(=O)=O

InChI

1S/C13H11N5O9S2.2K/c14-10-5-1-4(28(22,23)24)2-6-9(5)7(3-8(10)29(25,26)27)12(20)18(11(6)19)17-13(21)16-15;;/h1-3H,14-15H2,(H2,16,17,21)(H,22,23,24)(H,25,26,27);;/q;2*+1/p-2

InChI key

HCWYSCVNSCIZCN-UHFFFAOYSA-L

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일반 설명

Lucifer yellow CH is a fixable dye with an excitation and emission spectrum of 430 nm and 540 nm, respectively.[1]LuciferYellow CH (LYCH) is a small, hydrophilic molecule,[2] and highly fluorescent dye that is used for intracellular staining for the studies conducted on various types of cells.[3] It acts as a marker for the detection of well-established tight junctions[2] in smooth muscles, glia, and interstitial cells of Cajal (ICC).[3]

애플리케이션

Lucifer yellow CH dipotassium salt has been used:
  • to visualize cell morphology[1]
  • to assess intercellular communication activity through gap junction by scrape-loading assay[4][5]
  • as a component of the intracellular solution, which filled the dendrites for the visualization of neuronal morphology[6]
  • in the intracellular solution, to intracellularly label recorded neurons[7]

특징 및 장점

Highly fluorescent dye, useful in marking nerve cells.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

개인 보호 장비

Eyeshields, Gloves, type N95 (US)


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문서 라이브러리에서 최근에 구매한 제품에 대한 문서를 찾아보세요.

문서 라이브러리 방문

In vitro mesenchymal-epithelial transition in NIH3T3 fibroblasts results in onset of low-dose radiation hypersensitivity coupled with attenuated connexin-43 response
Mathur A, et al.
Biochimica et biophysica acta. General subjects, 1862(3), 414-426 (2018)
Zebrafish retinal slice preparation
Connaughton, VP
Methods in Cell Science : An Official Journal of the Society for In Vitro Biology, 25(1-2), 49-58 (2003)
Shuaiwei Wang et al.
Mediators of inflammation, 2020, 8094347-8094347 (2020-12-10)
Connexin (Cx) family members form hemichannels (HCs) and gap junctions (GJs). Biological functions of Cx HCs have not been adequately characterized due to the inability to selectively target HCs or GJs. Recently, we developed a 6-mer peptide mimetic (P5) of
Connexin-43 downregulation in G2/M phase enriched tumour cells causes extensive low-dose hyper-radiosensitivity (HRS) associated with mitochondrial apoptotic events
Ghosh S, et al.
Cancer letters, 363(1), 46-59 (2015)
Menachem Hanani
Journal of cellular and molecular medicine, 16(1), 22-31 (2011-07-12)
The fluorescent dye Lucifer yellow (LY) was introduced in 1978, and has been extremely useful in studying cell structure and communications. This dye has been used mostly for labelling cells by intracellular injection from microelectrodes. This review describes the numerous

문서

This is a protocol for a Membrane Integrity test for Lipid-PAMPA Artificial Membranes

질문

1–2 / 2 질문  
  1. What is the dye purity for product L0144?

    What is the dye purity for product L0144?

    1 답변
    1. At M-Clarity quality level 100, Product L0144 is evaluated based on appearance, infrared spectrum, solubility, and fluorescence specifications. Please refer to the Certificate of Analysis found on the DOCUMENTATION section of the Product Detail Page: https://www.sigmaaldrich.com/product/sigma/l0144#product-documentation

      Using the results on the specification sheet, dye content can be calculated from the testing performed to determine carbon and nitrogen levels.

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  2. What is the stability and shelf life for product L0144, lot SHBN6250 once it has been prepared in a solution?

    1 답변
    1. The product is soluble in water at a concentration of 1 mg/mL, producing a clear, yellow-orange colored solution. It is also soluble in water with heat at a concentration of 10 mg/mL, resulting in a slightly hazy orange colored solution. Additionally, a solution stability test revealed that a solution of L0144 in water at a concentration of 1.7 mg/mL, stored in the dark at room temperature for 32 days, showed no detectable change in the UV-VIS spectrum.

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