I1284

Isopropyl β-D-thiogalactopyranoside solution

ReadyMade IPTG solution for Blue-white screening

• 동의어(들): IPTG solution
• 실험식(Hill 표기법): C₉H₁₈O₅S
• CAS Number: 367-93-1
• Molecular Weight: 238.30
• Beilstein: 4631
• MDL number: MFCD00063273
• UNSPSC 코드: 12352200
• PubChem Substance ID: 24895928
• NACRES: NA.52

속성

• Grade: Molecular Biology
• Quality Level: 200
• 설명: non-ionic
• 양식: liquid
• 분자량: 238.3
• 배송 상태: dry ice
• 저장 온도: −20°C
• SMILES string: CC(C)S[C@@H]1O[C@H](CO)[C@H](O)[C@H](O)[C@H]1O
• InChI: 1S/C9H18O5S/c1-4(2)15-9-8(13)7(12)6(11)5(3-10)14-9/h4-13H,3H2,1-2H3/t5-,6+,7+,8-,9+/m1/s1
• InChI key: BPHPUYQFMNQIOC-NXRLNHOXSA-N

설명

일반 설명

Isopropyl thiogalactose (IPTG) is a sugar derivative widely used for the induction of the expression of recombinant proteins in E. coli. This expression system requires that the gene of interest is cloned in plasmids containing the Lac promoter. IPTG Ready Made is a 0.2 μm filter sterilized solution of 200 mM IPTG. This reagent remains liquid at its storage temperature (-20 °C), thus avoiding freeze-thaw cycles and providing a user-friendly solution.

애플리케이션

Isopropyl β-D-thiogalactopyranoside solution is:

• used as an inducer for the expression of single domain antibodies(sdAbs) in E coli[1] and bioactive peptides[2]
• used in its half-life and decay studies[3]
• suitable for induction of recombinant protein expression in systems controlled by the lac repressor

생화학적/생리학적 작용

Isopropyl β-D-thiogalactopyranoside binds to the lac repressor and releases the tetrameric repressor from the lac operator in an allosteric manner. This binding allows the transcription of genes in the lac operon, such as the gene coding for β--galactosidase. The β-galactosidase gene is transcribed and monosaccharides are the product. Since Isopropyl β-D-thiogalactopyranoside cannot be metabolized by E. coli its concentration remains constant. Therefore, it is not a variable in the experiment.[4]

원리

Isopropyl β-D-thiogalactopyranoside binds to the lac repressor and releases the tetrameric repressor from the lac operator in an allosteric manner. This binding allows the transcription of genes in the lac operon, such as the gene coding for β-galactosidase. The β-galactosidase gene is transcribed and monosaccharides are the product.

안전성 정보

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 2
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)