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Key Documents

D2532

Sigma-Aldrich

Denhardt′s Solution 50x

for Northern and Southern blotting, solution

동의어(들):

blocking reagent, blocking solution, denhardts, hybridization buffer, hybridization solution, northern blocking solution, pre-hybridization, southern blocking solution

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About This Item

UNSPSC 코드:
12161700
NACRES:
NA.25

Quality Level

형태

solution

기술

Southern blotting: suitable

외래 활성

DNAses, RNases, NICKases, endonucleases, and exonucleases, none detected

저장 온도

−20°C

일반 설명

Denhardt′s Solution is a blocking agent routinely used to reduce non-specific binding of detection reagents to the Northern and Southern Blot membranes. This 50x concentrate can be diluted to any concentration for use in a variety of pre-hybridization and hybridization buffers.

성분

Denhardt′s Solution is 1% Bovine Serum Albumin (BSA), 1 % Ficoll, and 1 % Polyvinylpyrrolidone (PVP) in water.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable


시험 성적서(COA)

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프로토콜

Ficoll 400® is a non-ionic polymer with high hydrophilicity and water solubility, suitable for various biological applications.

Learn Northern and Southern blotting basics, with protocols and applications for macromolecule transfer to membrane supports.

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