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문서

S0902

Sigma-Aldrich

SSC Buffer

for Northern and Southern blotting, powder blend

동의어(들):

Saline-sodium citrate buffer

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About This Item

UNSPSC 코드:
41105319
NACRES:
NA.25

형태

powder blend

pH

6.8-7.2 (20-25 °C, 263 mg/L in water)

solubility

water: 263 mg/mL, clear, colorless

외래 활성

DNAse, none detected
Endonuclease, none detected
Exonuclease, none detected
NICKase, none detected
RNAse, none detected

저장 온도

room temp

관련 카테고리

일반 설명

SSC Buffer 20x Powder is a standard reagent in Southern and Northern blotting procedures. This concentrated powder should be reconstituted in molecular biology grade water to the indicated final volume before use (final solution is a 20x). The 20x concentrate can be used straight or diluted to make SSC working solutions with concentrations as low as 0.5x.

애플리케이션

Saline-sodium citrate (SSC) Buffer has been used as a washing buffer in microarray technique. It has also been used as a constituent of fluorescence in situ hybridization (FISH) buffer.
Suitable for:
  • Preparing nucleic acids for hybridization
  • Hybridization buffer for Northern and Southern blots
  • Washing buffer for Northern and Southern blots

특징 및 장점

  • Multi-purpose use in hybridizations
  • Easily reconstituted and diluted to any concentration needed

성분

Final SSC Buffer 20x Concentrate contains 0.3 M sodium citrate in 3M NaCl.

관련 제품

제품 번호
설명
가격

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 1

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

시험 성적서(COA)

제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.

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프로토콜

Northern & Southern Blot Protocols

Learn Northern and Southern blotting basics, with protocols and applications for macromolecule transfer to membrane supports.

자사의 과학자팀은 생명 과학, 재료 과학, 화학 합성, 크로마토그래피, 분석 및 기타 많은 영역을 포함한 모든 과학 분야에 경험이 있습니다..

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