H7039

3-Hydroxy-3-methylglutaryl-CoA reductase human

solution

• 동의어(들): HMGCoA Reductase, HMGR
• 효소 위원회 번호: 1.1.1.34 (BRENDA, IUBMB)
• UNSPSC 코드: 12352204
• NACRES: NA.54

속성

• 분석: ≥90% (SDS-PAGE)
• Quality Level: 200
• 양식: solution
• 특이 활성도: 2-8 units/mg protein
• 분자량: ~76 kDa (for the fusion protein)
• UniProt 수납 번호: P04035
• 배송 상태: dry ice
• 저장 온도: −70°C
• 유전자 정보: human ... HMGCR(3156)

설명

애플리케이션

3-Hydroxy-3-methylglutaryl-CoA reductase human has been used:

• in anti-HMGCR enzyme linked immunosorbent assay (ELISA)[1]
• as an antigen to measure anti-HMGCR antibodies by blot analysis[2]
• in HMG-CoA reductase activity assays[3]
• as an antigen to compare in-house antigen via sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE)/western blot[4]

생화학적/생리학적 작용

3-Hydroxy-3-methylglutaryl-CoA reductase/HMGCR serves as a rate limiting enzyme in mevalonate pathway. It also controls the oncogenic Hippo transducers TAZ/YAP.[5] HMGCR also perform as a genetic modifier for Alzheimer′s disease (AD).[6]

Hydroxy-3-methylglutaryl-CoA reductase catalyzes the committed step in cholesterol biosynthesis.

물리적 특성

Consists of the HMG CoA reductase catalytic domain fused to GST protein.

단위 정의

One unit will convert 1.0 μmole of NADPH to NADP⁺ per minute at 37 °C.

물리적 형태

Supplied as a solution containing 250 μg protein in 50 mM Tris pH 7.5, with 5 mM DTT, 1:200 Protease Inhibitor Cocktail (Catalog Number P8340) and 50% (w/v) glycerol.

안전성 정보

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable