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Merck
모든 사진(5)

문서

H1029

Sigma-Aldrich

Monoclonal Anti-polyHistidine antibody produced in mouse

clone HIS-1, ascites fluid

동의어(들):

Monoclonal Anti-polyHistidine, Monoclonal 6 His epitope tag, Monoclonal 6xHis-tag, Monoclonal HHHHHH epitope tag, Monoclonal Hexa His tag, Monoclonal His-tag, Monoclonal His6 tag, Monoclonal Histidine tagged, Monoclonal Poly-His-tag

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모든 사진(5)

About This Item

UNSPSC 코드:
12352203
NACRES:
NA.56

생물학적 소스

mouse

Quality Level

200

결합

unconjugated

항체 형태

ascites fluid

항체 생산 유형

primary antibodies

클론

HIS-1, monoclonal

형태

liquid

기술

dot blot: suitable
immunoprecipitation (IP): suitable
indirect ELISA: suitable
western blot: 1:3,000 using lysates of Escherichia coli induced to express a 6xHis tagged protein

동형

IgG2a

배송 상태

dry ice

저장 온도

−20°C

타겟 번역 후 변형

unmodified

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일반 설명

The Monoclonal Anti-polyHistidine (mouse IgG2a) antibody recognizes native or denatured, reduced forms of synthetic polyhistidine and polyhistidine-tagged fusion proteins. The antibody preferentially recognizes the N-terminal tagged fusion protein and is reactive with fusion proteins expressed by the prokaryotic expression vectors pET, pRSET, and pTrc.

특이성

The antibody recognizes synthetic polyHistidine, as well as native or denatured, reduced forms of proteins tagged with 6X histidines, expressed in selected vectors.

면역원

recombinant polyHistidine tagged fusion protein.

애플리케이션

Antibody suitable for immunoblotting, dot blot assays, ELISA and immunocytochemistry. Monoclonal Anti-polyHistidine antibody produced in mouse has been used in Western blotting.

생화학적/생리학적 작용

The IgG2a (Immunoglobulin G 2a) gene actively takes part in stimulating autoimmunity in mice. The gene is found to be highly expressed in virus infection.

물리적 형태

Suppled as ascites fluid with 15mM sodium azide.

기타 정보

Recommended dilution for immunoblotting is 1:3000

법적 정보

This Product is covered by patent DE 19507166 and foreign equivalents exclusively licensed to QIAGEN GmbH, Qiagen Strasse 1, D-40724 Hilden, Germany.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 1

시험 성적서(COA)

제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.

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문서 라이브러리 방문

Contribution of IRF5 in B cells to the development of murine SLE-like disease through its transcriptional control of the IgG2a locus.
Savitsky D A, et al.
Proceedings of the National Academy of Sciences of the USA (2010)
Ora Hazak et al.
PLoS biology, 17(7), e3000085-e3000085 (2019-07-12)
Signaling cross talks between auxin, a regulator of plant development, and Ca2+, a universal second messenger, have been proposed to modulate developmental plasticity in plants. However, the underlying molecular mechanisms are largely unknown. Here, we report that in Arabidopsis roots
Association of a novel pre-ribosomal complex in Trypanosoma brucei.
Wang L
Eukaryotic Cell (2013)
G protein-coupled receptor-promoted trafficking of G?1?2 leads to AKT activation at endosomes via a mechanism mediated by G?1?2-Rab11a interaction.
Garcia-Regalado A, et al.
Molecular Biology of the Cell, 19(10), 4188-4200 (2008)
A variant upstream of IFNL3 (IL28B) creating a new interferon gene IFNL4 is associated with impaired clearance of hepatitis C virus.
Prokunina-Olsson L, et al.
Nature Genetics, 45(2), 164-164 (2013)
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