H5666

Granulocyte-Macrophage Colony-Stimulating Factor human

GM-CSF, recombinant, expressed in HEK 293 cells, HumanKine^®, suitable for cell culture

• 동의어(들): GM-CSF human
• MDL number: MFCD00166611
• UNSPSC 코드: 12352202
• NACRES: NA.77

속성

• 생물학적 소스: human
• Quality Level: 100
• 재조합: expressed in HEK 293 cells
• 분석: ≥95% (SDS-PAGE)
• 형태: lyophilized powder
• 효능: ≤5 ng/mL EC₅₀
• 품질: endotoxin tested
• 분자량: dimer 15-36 kDa (glycosylated)
• 포장: pkg of 5X10 μg; pkg of 10 μg
• 저장 조건: avoid repeated freeze/thaw cycles
• 기술: cell culture | mammalian: suitable
• 불순물: ≤1 EU/mg
• UniProt 수납 번호: P04141
• 저장 온도: −20°C
• 유전자 정보: human ... CSF2(1437)

설명

일반 설명

Granulocyte-Macrophage Colony-Stimulating Factor is mapped to human chromosome 5q21-q32.

애플리케이션

Granulocyte-Macrophage Colony-Stimulating Factor human has been used as a colony-stimulating cytokine in human acute myeloid leukemia cell line (OCI-M2) to test its effect on 5-azacitidin (AZA)-mediated DNA demethylation of upstream regulatory element (URE).

생화학적/생리학적 작용

Granulocyte-Macrophage Colony-Stimulating Factor (GM-CSF) is an immune modulator and proinflammatory cytokine. It binds to the heteromeric cell-surface receptors present in the immune cells like granulocytes, endothelial cells and macrophages. GM-CSF is implicated in inflammation associated disorder of the lung and renal system.

Granulocyte-macrophage colony stimulating factor (GM-CSF) is a growth and differentiation factor for cells in the granulocyte, macrophage and eosinophil lineage. GM-CSF stimulates colony formation from pluripotential progenitor cells at extremely low concentrations and is an essential survival and proliferative factor for hematopoietic progenitor cells in all divisions up to maturity. It also stimulates growth in some epithelial cells and osteoclasts. GM-CSF is produced by a variety of cell types (monocytes, endothelial cells, T-cells, fibroblasts, mitogen-stimulated B-cells, and LPS-stimulated macrophages). GM-CSF is secreted as a single chain glycoprotein containing 128 amino acids for human with a conserved disulfide bond. Human and murine GM-CSF share approx. 54% sequence homology and do not cross-react in bioactivity.

주의사항

Although human and mouse GM-CSF share 54% amino acid sequence homology, their biological actions are species-specific and exhibit no cross-species reactivity.

물리적 형태

Lyophilized from a 0.2 μm filtered solution of 1x PBS.

제조 메모

HumanKine GM-CSF is expressed as a 15-36 kDa glycosylated monomer in human HEK 293 cells. Production in human HEK 293 cells offers authentic glycosylation. Glycosylation contributes to stability in cell growth media and other applications. Although human and mouse GM-CSF share 54% amino acid sequence homology, their biological actions are species-specific and exhibit no cross-species reactivity.

분석 메모

The specific activity was determined by the dose-dependent stimulation of the proliferation of human TF-1 cells (human erythroleukemic indicator cell line).

법적 정보

HumanKine is a registered trademark of Proteintech Group, Inc. and Humanzyme, Inc

안전성 정보

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable