G0407

Granulocyte colony-stimulating factor human

G-CSF, recombinant, expressed in E. coli, suitable for cell culture

• MDL number: MFCD00166475
• UNSPSC 코드: 12352202
• NACRES: NA.77

속성

• 생물학적 소스: human
• Quality Level: 100
• 재조합: expressed in E. coli
• 분석: ≥95% (SDS-PAGE)
• 형태: lyophilized powder
• 효능: 0.01-0.1 ng/mL
• 품질: endotoxin tested
• 분자량: 18.8 kDa (175 amino acids including N-terminal methionine)
• 포장: pkg of 5 and 25 μg
• 기술: cell culture | mammalian: suitable
• 불순물: <1 EU/μg (LAL test)
• UniProt 수납 번호: P09919
• 저장 온도: −20°C
• 유전자 정보: human ... CSF3(1440)

설명

일반 설명

Granulocyte colony-stimulating factor human (GCSF) gene is mapped to human chromosome 17q21.1. Structurally, GCSF comprises a four α-helical bundle.

애플리케이션

Granulocyte colony-stimulating factor human has been used:

• in culturing human neutrophils
• as a growth factor component of double layer granulocyte-macrophage colony-forming unit (CFU-GM) culture system for CD34⁺ cells
• to test its protective effects chronic liver damage model

생화학적/생리학적 작용

Granulocyte colony-stimulating factor human (GCSF) interacts specifically with the immunoglobin (Ig) domain of the granulocyte colony-stimulating factor receptor (GCSFR).

Granulocyte-colony stimulating factor (G-CSF) is a proliferation, differentiation, survival, and activation factor for hematopoietic cells of the restricted neutrophilic granulocyte lineage. It is produced by macrophages activated by endotoxin (LPS), by monocytes activated by TNF-α with IFNγ, by fibroblasts and endothelial cells activated by IL-1 or TNF-α, and by bone marrow stromal cells activated by IL-1 or LPS. It is suggested that during the inflammatory process endotoxins stimulate tissue macrophages to produce not only G-CSF but several other cytokines, including IL-1 and TNF-α, which in turn stimulate more G-CSF release from endothelial cells and fibroblasts. G-CSF can also synergize with IL-3 to shorten the G₀ period of early hematopoietic progenitors. In addition to the namesake proliferative activity, G-CSF acts on mature neutrophils to enhance their survival and to stimulate their tumorcidal activity. Human G-CSF binds and activates a 130 kD to150 kD glycoprotein single chain receptor that has been classified as a member of the hematopoietic (cytokine) receptor family, the cytokine receptor class I, or the gp130 related cytokine receptor family (although it does not apparently bind to gp130). G-CSF receptors can be found on neutrophils, myeloid leukemia cells that respond to G-CSF, bone marrow cells of neutrophilic granulocyte lineage, and on placental trophoblasts, plus a soluble form may be expressed. Two forms of human G-CSF (177 and 174 amino acids) are synthesized from a single gene by alternative splicing, but murine G-CSF is a single expressed form of 178 amino acids. Human and murine G-CSF share 73% amino acid sequence homology and full cross-reactivity.

물리적 형태

Lyophilized from 0.2 μm-filtered solution of 10 mM HOAc containing 50 μg BSA per μg G-CSF

분석 메모

The biological activity is measured in a cell proliferation assay using a murine myeloblastic cell line, NFS-60.

안전성 정보

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: Eyeshields, Gloves, type N95 (US)