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Merck
모든 사진(1)

문서

H1404

Sigma-Aldrich

Hepatocyte Growth Factor human

HGF, recombinant, expressed in Baculovirus infected High-5 cells, suitable for cell culture

동의어(들):

HGF, Hepatopoietin A, Scatter Factor

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About This Item

EC Number:
MDL number:
UNSPSC 코드:
12352202
NACRES:
NA.77

생물학적 소스

human

Quality Level

재조합

expressed in Baculovirus infected High-5 cells

분석

>95% (SDS-PAGE)

형태

lyophilized powder

효능

1.0-20.0 ng/mL

품질

endotoxin tested

분자량

~80 kDa

포장

pkg of 5x5 μg
pkg of 5 μg

저장 조건

avoid repeated freeze/thaw cycles

기술

cell culture | mammalian: suitable

불순물

<1.0 EU/μg

UniProt 수납 번호

저장 온도

−20°C

유전자 정보

human ... HGF(3082)

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생화학적/생리학적 작용

Hepatocyte growth factor (HGF) is a potent mitogen for hepatocytes and a variety of cell types including endothelial and epithelial cells, melanocytes, and keratinocytes. It also mediates epithelial morphogenesis. HGF is the ligand for the tyrosine kinase receptor encoded by the met proto-oncogene.

기타 정보

The NSO-expressed recombinant HGF is greater than 95% heterodimeric HGF. The insect cell line-expressed recombinant HGF is a mixture of single chain HGF (predominant) with some heterodimeric HGF. Single chain HGF and heterodimeric HGF are equally active in in vitro assays.

물리적 형태

Lyophilized from a 0.2 μm filtered solution in 20 mM NaH2PO4 and 500 mM NaCl, pH 7.0 with 50 μg BSA per 1 μg HGF

제조 메모

Reconstitute at 50 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin. After reconstitution, this solution can be stored at 2-8 °C for up to one month or at -20 °C to -70 °C for up to three months. Us a manual defrost freezer and avoid repeated freeze-thaw cycles.

분석 메모

The biological activity is measured by its ability to induce IL-11 secretion by Saos-2 human osieosarcoma cells.

Storage Class Code

11 - Combustible Solids

WGK

WGK 2

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

개인 보호 장비

dust mask type N95 (US), Eyeshields, Gloves


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문서

Hepatocyte growth factor (HGF) is a potent mitogen for hepatocytes and a variety of other cells, including endothelial and epithelial cells, melanocytes, and keratinocytes.

자사의 과학자팀은 생명 과학, 재료 과학, 화학 합성, 크로마토그래피, 분석 및 기타 많은 영역을 포함한 모든 과학 분야에 경험이 있습니다..

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