For enzymatic determination of L-glutamine and L-glutamate via reduction of NAD to NADH, measured spectrophotometrically at 339 nm. The detection ranges for this kit are: 0.056 - 0.56 mM L-glutamate; 0.14 - 1.4 mM L-glutamine.
Glutamine and Glutamate Determination Kit has been used to determine the concentration of L-glutamine:
The Glutamine/Glutamate Determination Kit is designed for the spectrophotometric measurement of L-glutamine and/or L-glutamate via enzymatic deamination of L-glutamine and dehydrogenation of L-glutamate with conversion of NAD+ to NADH. This kit may be used for the spectrophotometric measurement of L-glutamine and/or L-glutamate in liquid preparations.
Glutamine metabolism plays an important role in cancer development and progression. Glutaminase C (GAC), the first enzyme in glutaminolysis, has emerged as an important target for cancer therapy and many studies have focused on the mechanism of enhanced GAC expression
Glycolysis and glutaminolysis are heavily involved in the metabolic reprogramming of cancer cells. The activation of oncogenes and inactivation of tumor suppressor genes has a marked effect on the cellular metabolic processes glycolysis and glutaminolysis. N-Myc downstream-regulated gene 2 (NDRG2)
Monitoring for overreaching in rugby league players
Coutts AJ, et al.
European Journal of Applied Physiology, 99(3), 313-324 (2007)
Clinical and experimental dermatology, 42(6), 614-621 (2017-06-10)
Malignant melanoma (MM) is an aggressive malignancy, which accounts for 80% of skin cancer-related deaths and is notably resistant to conventional chemotherapeutic agents. One of the most common treatments for melanoma is surgery, followed by various combinations of chemotherapy drugs.
Gankyrin plays important roles in tumorigenicity and metastasis of hepatocellular carcinoma (HCC). We have for the first time investigated the effects of Gankyrin on glycolysis and glutaminolysis both in vitro and in vivo, including in patient-derived xenografts. We reported Gankyrin