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GE29-0915-96

Superose^® 6 Increase 10/300 GL

Name: Superose<SUP>®</SUP> 6 Increase 10/300 GL
Brand: SIGMA

Cytiva 29-0915-96, 10/300 GL

동의어(들):

Superose^® 6 Increase

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About This Item

UNSPSC 코드:

41115700

NACRES:

SB.52

추천 제품

Slide 1 of 7

1 of 7

EP5384000012

Eppendorf^® ThermoMixer^® F1.5

Supelco

CL4B200

Sepharose^™ CL-4B

Millipore

UFC9030

Amicon^® Ultra Centrifugal Filter, 30 kDa MWCO

Sigma-Aldrich

S200HR

Sephacryl^®

Millipore

UFC9010

Amicon^® Ultra Centrifugal Filter, 10 kDa MWCO

Sigma-Aldrich

S9514

Superose^® 12 Prep Grade

Millipore

UFC9003

Amicon^® Ultra Centrifugal Filter, 3 kDa MWCO

material

glass column
polyethylene filter

제품 라인

Superose^®

분자량

5000-5,000,000 Da (globular proteins)

포장

package of 1 ea

제조업체/상표

Cytiva 29-0915-96

파라미터

<0.35 mL/min flow rate
<0.75 mL/min flow rate (20% ethanol at room temp)
<0.75 mL/min flow rate
<1.50 mL/min flow rate (water at room temp)
25-500 μL sample volume
3 Mpa

베드 용적

24 mL

기질

cross-linked agarose
polymeric

기질 활성군

agarose phase

cleaning

1-14

working range

3-12

분리 기술

size exclusion (SEC)

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일반 설명

Superose^® 6 Increase 10/300 GL is a versatile, prepacked column for high-resolution size exclusion chromatography in small-scale (< 500 μl sample volume) preparative purification, as well as for characterization and analysis of proteins and other biomolecules with molecular weights between 5 000 and 5 000 000.

특징 및 장점

Increased resolution compared with Superose 6, for higher purity and improved analysis results.
Reduced runtime compared with Superose 6, for rapid results.
Versatile use for both preparative and analytical purposes, especially for large proteins and complexes.
Tolerance to repeated harsh cleaning procedures at high pH, giving long column lifetime and minimal carry-over.

적합성

Stable in all common buffers: 1 M acetic acid, 8 M urea, 6 M guanidine hydrochloride, 1% SDS, organic solvents, 0.5 M NaOH (for cleaning-in-place)

Superose^® 6 Increase 10/300 GL column is suitable for small-scale (25 – 500 μl sample volume) preparative purification for obtaining milligram amounts of highly size-homogenous target proteins at the polishing stage of a purification procedure. It is also well-suited for the characterization of large protein complexes and for the study of protein-protein interactions.

저장 및 안정성

Store at 4 to 30 °C (20% Ethanol)

분석 메모

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

법적 정보

Superdex is a registered trademark of Cytiva

Superose is a registered trademark of Cytiva

Storage Class Code

3 - Flammable liquids

시험 성적서(COA)

제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.

이 제품을 이미 가지고 계십니까?

문서 라이브러리에서 최근에 구매한 제품에 대한 문서를 찾아보세요.

문서 라이브러리 방문

Chemical Cross-Linking Enables Drafting ClpXP Proximity Maps and Taking Snapshots of In Situ Interaction Networks.

Anja Fux et al.

Cell chemical biology, 26(1), 48-59 (2018-11-13)

Detection of dynamic protein-protein interactions within complexes and networks remains a challenging task. Here, we show by the example of the proteolytic ClpXP complex the utility of combined chemical cross-linking and mass spectrometry (XL-MS) to map interactions within ClpP and

문서

Setting Pressure Limits for Size Exclusion Chromatography

Understanding pressure drop in chromatography systems is crucial for optimal functionality across different parts of the system.

프로토콜

Performing a Separation with Superose

Superose from Cytiva are Size Exclusion Chromatography media consisting of a composite base matrix of dextran and agarose. This page shows how to perform a separation with a superose column.

Sample Preparation for Size Exclusion Chromatography

Ensure chromatographic sample clarity to avoid column clogging, reduce washing needs, and prolong column life for purification.

문서

Superose^® 6 Increase 10/300 GL