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Merck
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문서

S200HR

Sigma-Aldrich

Sephacryl®

200-HR, MW range 5-250 kDa (globular proteins), MW range 1-80 kDa (dextrans)

동의어(들):

Poly([allyl dextran]-co-N,N′-methylenebisacrylamide)

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About This Item

CAS Number:
MDL number:
UNSPSC 코드:
12352202
NACRES:
NA.56

설명

stability: stable to 0.2M NaOH

형태

suspension (20% aqueous ethanol)

특징

autoclavable

기질

crosslinked allyl dextran/N,N′-methylenebisacrylamide copolymer

입자 크기

25-75 μm (wet)

공극 크기

1-80 kDa MW range (dextrans)
5-250 kDa MW range (globular proteins)

호환성

mode of use size exclusion chromatography

저장 온도

2-8°C

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관련 카테고리

일반 설명

  • Supplied swollen, suspended in 20% aqueous ethanol
  • Autoclavable
  • Stable to 0.2N sodium hydroxide (NaOH)
  • Store at refrigerated temperature (2-8 °C)
S200HR-750ML′s updated product number is GE17-0584-01

애플리케이션

Sephacryl® is used in gel filtration chromatography, protein chromatography, and resins and separation media. Sephacryl® has been used to develop a novel high-throughput screening assay for sickle cell disease drug discovery. Sephacryl® has also been used to purify and characterize ovine pancreatic elastase as well as a lysozyme from human urine.
For size exclusion chromatography; the HR grade is a smaller particle size with narrower size range distribution, allowing for more efficient separations at faster flow rates. Recommended fractionation ranges are listed for globular proteins and dextrans..

법적 정보

Sephacryl is a registered trademark of Cytiva

관련 제품

픽토그램

Flame

신호어

Warning

유해 및 위험 성명서

Hazard Classifications

Flam. Liq. 3

Storage Class Code

3 - Flammable liquids

WGK

WGK 1

Flash Point (°F)

100.4 - 109.4 °F - closed cup

Flash Point (°C)

38 - 43 °C - closed cup

개인 보호 장비

Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter


시험 성적서(COA)

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문서 라이브러리 방문

Andor J Kiss et al.
The Journal of experimental biology, 207(Pt 26), 4633-4649 (2004-12-08)
The eye lenses of the Antarctic nototheniid fishes that inhabit the perennially freezing Antarctic seawater are transparent at -2 degrees C, whereas the cold-sensitive mammalian and tropical fish lenses display cold-induced cataract at 20 degrees C and 7 degrees C
Zhili Xu et al.
Methods in enzymology, 468, 147-165 (2009-01-01)
Chemical probing is widely used as a rapid approach for assessing RNA structure, folding, and function. In this chapter, we outline procedures for handling and using chemicals commonly used to probe nucleic acids. Detailed experimental conditions and design for footprinting
Valerie Waters et al.
American journal of respiratory and critical care medicine, 183(5), 635-640 (2010-10-05)
Stenotrophomonas maltophilia is one of the more common multidrug-resistant organisms isolated from the respiratory tract of patients with cystic fibrosis (CF), but the effect of chronic S. maltophilia infection on CF lung disease is unknown. To determine the impact of
M Hamilton et al.
The Journal of biological chemistry, 276(31), 29079-29090 (2001-05-19)
Phorbol ester stimulation of the MAPK cascade is believed to be mediated through the protein kinase C (PKC)-dependent activation of Raf-1. Although several studies suggest that phorbol ester stimulation of MAPK is insensitive to dominant-negative Ras, a requirement for Ras
Alberto Cruz Rodríguez et al.
Molecules (Basel, Switzerland), 25(7) (2020-04-12)
The presence of isoforms of β-glucosidase has been reported in some grasses such as sorghum, rice and maize. This work aims to extract and characterize isoform II in β-glucosidase from S. edule. A crude extract was prepared without buffer solution

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