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Merck
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주요 문서

UFC9003

Millipore

Amicon® Ultra Centrifugal Filter, 3 kDa MWCO

greener alternative

sample volume 15 mL, regenerated cellulose membrane, MWCO 3 kDa

동의어(들):

Centrifugal concentrator with Ultracel® membrane,3 kDa MWCO, 15 mL sample volume

로그인조직 및 계약 가격 보기

크기 선택

8 PKG
₩285,114
24 PKG
₩580,479
96 PKG
₩2,298,975

₩285,114


예상 입고일2025년 4월 21일세부사항


벌크 견적 요청

크기 선택

보기 변경
8 PKG
₩285,114
24 PKG
₩580,479
96 PKG
₩2,298,975

About This Item

UNSPSC 코드:
41104916
eCl@ss:
36100101
NACRES:
NB.24

₩285,114


예상 입고일2025년 4월 21일세부사항


벌크 견적 요청

material

polyethylene cap
polyethylene liner
polypropylene tube
regenerated cellulose membrane
regenerated cellulose support
styrene-butadiene housing

Quality Level

무균

non-sterile

제품 라인

Amicon®

포장

pkg of 24 pkg
pkg of 8 pkg
pkg of 96 pkg

제조업체/상표

Amicon® Ultra

sustainability

Greener Alternative Product

파라미터

15 mL sample volume

기술

protein extraction: suitable
protein purification: suitable

길이

12.1 cm (4.807 in.)

직경

2.97 cm

여과 면적

7.6 cm2

용적

15 mL

작업 용적

≤15 mL
15 mL

공극 크기

3 kDa MWCO

환경친화적 대안 카테고리

배송 상태

ambient

일반 설명

Amicon® Ultra 15 mL centrifugal devices with Ultracel® 3,000 Dalton molecular weight cutoff filters provide fast ultrafiltration, and reliable retention of macromolecules. For example, Cytochrome C (12.4 kDa) at 0.25 mg/ml initial concentration is retained at >90% .

The vertical design and available membrane surface area provide fast sample processing, high sample recovery (typically greater than 90% of dilute starting solution), and the capability for 80-fold concentration. Solute polarization and subsequent fouling of the membrane are minimized by the vertical design, and a physical deadstop in the filter device prevents spinning to dryness and potential sample loss.

The concentrate is collected from the filter device sample reservoir using a pipetter, while the ultrafiltrate is collected in the provided centrifuge tube. The device can be spun in a swinging bucket or fixed angle rotor. With their vertical membrane design and deadstop, Amicon® Ultra 15 mL filters deliver the highest recovery with short spin times.
We are committed to bringing you Greener Alternative Products, which have been re-engineered to enable more sustainable research solutions. The inner filtration device is made from 60% bio-circular material allocated using the ISCC mass balance approach. Click here for more information.

애플리케이션

  • Ultrafiltration with 3,000 Dalton molecular weight cutoff Ultracel® membrane.
  • Concentration of biological samples containing antigens, antibodies, enzymes, nucleic acids, or microorganisms.
  • Purification of macromolecular components found in tissue culture extracts or cell lysates and protein removal prior to HPLS.
  • Concentration of dilute or pre-purified proteins from column eluents.
  • Desalting, buffer exchange and protein dialysis.

특징 및 장점

  • Ultracel® regenerated cellulose membrane with 3 kDa NMWCO delivers retentate recovery of greater than 90%.
  • Vertical membrane reduces concentration polarization for ultra-fast spin times (as fast as 10–15 minutes).
  • Heat-sealed membrane minimizes downstream extractables.
  • 100% integrity tested for reliable performance.
  • Convenient sample monitoring with translucent housing and volume gradations.
  • Direct pipettor sample access eliminates processing step to recover concentrate.
  • High concentration factors of 80–100X.

물리적 형태

Color Code: Clear with pink cap

법적 정보

Amicon is a registered trademark of Merck KGaA, Darmstadt, Germany
ULTRACEL is a registered trademark of Merck KGaA, Darmstadt, Germany

시험 성적서(COA)

제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.

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문서 라이브러리에서 최근에 구매한 제품에 대한 문서를 찾아보세요.

문서 라이브러리 방문

문서

나노입자 정제 및 생산에서 여과 방법 및 멤브레인 필터 선택.

Filtration methods and membrane filter selection in nanoparticle purification and production.

Explore ultrafiltration membrane principles and benefits for protein sample preparation, including solvent exchange and sample concentration.

질문

1–7 / 7 질문  
  1. Can we reuse the tube or it is single time use only?

    1 답변
    1. This product is intended for single use. Repeated usage may result in declining performance.

      도움이 되었습니까?

  2. Hello, I did not get if all the proteins will remain in the filter and if I can recover them from the filter (product: UFC9003 Millipore Amicon® Ultra Centrifugal Filter, 3 kDa MWCO). What will exactly remain in the liquid part in the bottom? Many thanks

    1 답변
    1. These centrifugal devices separate constituents of a given sample by molecular weight. This product has a molecular weight cut-off (MWCO) of 3000 Daltons. Components of the sample with a molecular weight greater that the designated 3 kDa will remain in the retained portion following centrifugation, while components of less that 3 kDa will filter through. See the image below to review this 2 part device.
      Please see the following link to review the product User Guide:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/251/395/pr05035w-ug-ms.pdf

      The link below is a video demonstrating the practical use of the Amicon products:
      https://www.youtube.com/watch?v=7uUUYqIpI6U

      도움이 되었습니까?

  3. Hi, I am writing to ask if you have centrifugal filters with hydrophobic membranes. I am looking to test them for oil/water separation downstream to a fermentation process for food applications.

    1 답변
    1. The ultrafiltration membranes used in Amicon devices are all hydrophilic. There are no hydrophobic options.

      도움이 되었습니까?

  4. Even sanitizing the devices with 70% ethanol, how many times may I use one tube? I mean, How many times can be used it?

    1 답변
    1. The unit is for one-time use only.

      도움이 되었습니까?

  5. How many tubes are inside the pack? For example, there is a pack size of 8 pkg. What does it mean? Does it mean 8 tubes or 8 packs of tubes? Does it mean that there are 8 tubes in the pack? Can you explain more, please?

    1 답변
    1. The package quantity for each item is as follows:
      UFC900308 - 8 tubes
      UFC900324 - 24 tubes
      UFC900396 - 96 tubes

      도움이 되었습니까?

  6. How much g (rcf) force can be applied during the spin?

    1 답변
    1. Amicon® Ultra-15 Centrifugal Filter Devices can be spun at 4,000 x g maximum for approximately 15-60 minutes when using a swinging-bucket rotor, or at 5,000 x g maximum for approximately 15-60 minutes when using a fixed-angle rotor.

      도움이 되었습니까?

  7. Is autoclave sterilization possible?

    1 답변
    1. The Amicon Ultra devices are manufactured under very thorough and controlled cleaning conditions, however they are not sterile and cannot be sterilized by UV or autoclaving. However, it is possible to sanitize the devices with 70% ethanol, followed by several rinses with sterile water or culture medium.

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