추천 제품
ligand
protein G
포장
pack of 1 × 500 μL
제조업체/상표
Cytiva 28-9440-08
저장 조건
(20% Ethanol, 20% medium slurry)
파라미터
Room temperature temp. range
기질
paramagnetic, spherical, highly cross-linked agarose particles
용량
13- 22 mg binding capacity(human IgG/ml medium)
저장 온도
2-8°C
일반 설명
Protein G Mag Sepharose™ are magnetic beads designed to simplify enrichment of target proteins by immunoprecipitation applications. Based on Sepharose™ with protein G as ligand. Protein G has high affinity for the Fc region of IgG antibodies.
애플리케이션
Protein G Mag Sepharose™ are magnetic beads designed to simplify enrichment of target Proteins by immunoprecipitation or pull-down applications. The magnetic beads are based on Sepharose™ with Protein G as ligand. Protein G has high affinity for the Fc region of IgG antibodies from a broad variety of species.
There are two protocols available for Protein enrichment: the cross-link protocol and the classic protocol. The protocols are simple with elution conditions optimized for both electrophoresis and MS analysis.
Protein G Mag Sepharose™ combines well-proven enrichment methods with the magnetic beads platform, which has excellent properties for small-scale sample preparation. Together with MagRack 6, a separation tool for six microcentrifuge tubes, up to six samples can be processed in parallel.
There are two protocols available for Protein enrichment: the cross-link protocol and the classic protocol. The protocols are simple with elution conditions optimized for both electrophoresis and MS analysis.
Protein G Mag Sepharose™ combines well-proven enrichment methods with the magnetic beads platform, which has excellent properties for small-scale sample preparation. Together with MagRack 6, a separation tool for six microcentrifuge tubes, up to six samples can be processed in parallel.
특징 및 장점
- Visible and dense Sepharose™ based magnetic beads with Protein A ligand, easy to spot and collect bound target Protein
- Non-adherent beads eliminate smearing effects and aggregate formation. Can be used without detergents
- Simple capture of target Protein in small or large sample volumes (Low microliter to high milliliter scale)
- Optimized capacity for enrichment/immunoprecipitation of target Proteins - only Low amounts of antibody needed
- Flexible protocols with elution conditions optimized for both electrophoresis and MS analysis
저장 및 안정성
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
분석 메모
To view the Certificate of Analysis for this product, please visit www.cytiva.com.
법적 정보
Sepharose is a trademark of Cytiva
신호어
Warning
유해 및 위험 성명서
Storage Class Code
3 - Flammable liquids
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
Structural insights into Siglec-15 reveal glycosylation dependency for its interaction with T cells through integrin CD11b.
Nature Communications, 14, 3496-3496 (2023)
문서
This page describes immunoprecipitation (immunoaffinity or pull-down techniques).
프로토콜
Protein A Mag Sepharose® Xtra and Protein G Mag Sepharose® Xtra enable high-capacity small-scale antibody purification and screening.
자사의 과학자팀은 생명 과학, 재료 과학, 화학 합성, 크로마토그래피, 분석 및 기타 많은 영역을 포함한 모든 과학 분야에 경험이 있습니다..
고객지원팀으로 연락바랍니다.