콘텐츠로 건너뛰기
Merck
모든 사진(1)

주요 문서

GE28-9365-50

GSTPrep™ Fast Flow 16/10

Cytiva 28-9365-50

로그인조직 및 계약 가격 보기
모든 사진(1)

About This Item

UNSPSC 코드:
13111000
NACRES:
NA.56
가격 및 재고 정보를 현재 이용할 수 없음 고객지원팀으로 연락바랍니다.

ligand

glutathione and 10-carbon linker arm

포장

pkg of 20 mL

제조업체/상표

Cytiva 28-9365-50

파라미터

<10 mL/min flow rate
1.5 bar (22 psi) (Over the Packed Bed During Operation)
22 psi

베드 크기

16 mm × 100 mm

베드 용적

20 mL

컬럼 I.D.

16 mm

Matrix

highly cross-linked 4% agarose

평균 직경

90 μm

cleaning

3-12

작동 범위

3-12

용량

>10 mg/mL binding capacity

관련 카테고리

일반 설명

GSTPrep™ FF 16/10 is prepacked, ready-to-use column containing Glutathione Sepharose™ 4 Fast FLow designed for one-step, scale-up purification of glutathione S-transferase (GST)-tagged proteins.
The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

애플리케이션

Glutathione Sepharose™ 4 Fast FLow is for scale-up purification of recombinant GST-tagged Proteins, other glutathione S-transferases, and glutathione-dependent Proteins. Mild elution conditions preserve Protein antigenicity and function.

특징 및 장점

  • Fast and easy one-step, scale-up purification of GST-tagged Proteins.
  • Binds approx. 200 mg recombinant GST/column
  • Mild elution conditions preserving Protein antigenicity and function.
  • Simple operation with a pump or chromatography system, such as ÄKTA® design.

저장 및 안정성

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)

분석 메모

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

기타 정보

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

법적 정보

GSTPrep is a trademark of Cytiva
Sepharose is a trademark of Cytiva
ÄKTA is a registered trademark of Cytiva

픽토그램

Flame
GHS02

신호어

Warning

유해 및 위험 성명서

H226

Storage Class Code

3 - Flammable liquids

가장 최신 버전 중 하나를 선택하세요:

시험 성적서(COA)

Lot/Batch Number

It looks like we've run into a problem, but you can still download Certificates of Analysis from our 문서 section.

도움이 필요하시면 연락하세요. 고객 지원 부서

이 제품을 이미 가지고 계십니까?

문서 라이브러리에서 최근에 구매한 제품에 대한 문서를 찾아보세요.

문서 라이브러리 방문

문서

Cleavage and Purification of GST-Tagged Protein Eluted from GSTrap

This page shows how to cleave and purify GST-tagged proteins eluted from GSTrap from Cytiva.

Cleavage and Purification of GST-Tagged Protein Bound to GSTrap

This page shows how to cleave and purify GST-tagged proteins bound to GSTrap from Cytiva.

Troubleshooting Purification Methods

This page shows troubleshooting methods for purification of GST-tagged proteins using Cytiva products.

Purifcation or Removal of Serine Proteases, e.g. Thrombin and Trypsin, and Zymogens

This page shows how to remove serine proteases, e.g. thrombin and trypsin, and zymogens from recombinant products using HiTrap Benzamidine FF and Benzamidine Sepharose 4 Fast Flow from Cytiva.

모두 보기

프로토콜

Removal of GST Tag by Enzymatic Cleavage

This page how to remove GST tags by enzymatic cleavage using Cytiva products.

Separation with Cytiva Products (GST Fusion Proteins)

Affinity chromatography tags like GST fusion proteins facilitate separations in various purification products from Cytiva.

GSTrap™ Columns for Purification

GSTrap™ affinity columns facilitate protein purification with Glutathione Sepharose® chromatography media for efficient purification processes.

Buffer Exchange and Desalting for Affinity Chromatography

How to perform buffer exchange and desalting with Sephadex G-25, HiTrap Desalting columns, or ÄKTAprime plus.

자사의 과학자팀은 생명 과학, 재료 과학, 화학 합성, 크로마토그래피, 분석 및 기타 많은 영역을 포함한 모든 과학 분야에 경험이 있습니다..

고객지원팀으로 연락바랍니다.