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ligand
recombinant protein A (E. coli)
포장
pack of 25 mL
제조업체/상표
Cytiva 17-1279-02
저장 조건
(20% Ehtanol)
Matrix
4% cross-linked agarose
입자 크기
60-165 μm
평균 직경
90 μm (d50v)
cleaning in place
2-11
작동 범위
3-10
용량
~50 mg binding capacity (human lgG/ml medium)
적합성
suitable for bioprocess medium
저장 온도
2-8°C
관련 카테고리
일반 설명
rProtein A Sepharose 4 Fast Flow is an affinity resin for antibody purification at laboratory and process scale.
Monoclonal and polyclonal antibody purification
rProtein A Sepharose 4 Fast Flow is built upon the well-established Sepharose Fast Flow platform and is used in many approved monoclonal antibody processes. It is composed of recombinant protein A coupled to the cross-linked 4% agarose base matrix.The highly crosslinked agarose resin enables high flow rates and high productivity.
Protein A resin characteristics
The recombinant protein is produced in E. coli and engineered with an oriented coupling to enhance binding capacity. Epoxy-based coupling ensures low ligand leakage. Specificity of the recombinant protein for the Fc region of IgG is similar to the native protein, giving a high degree of selectivity and yield.
Streamlined scale up
Scale-up with this resin is straightforward and is further aided by a range of prepacked process development tools, making purification scale-up straightforward.
Monoclonal and polyclonal antibody purification
rProtein A Sepharose 4 Fast Flow is built upon the well-established Sepharose Fast Flow platform and is used in many approved monoclonal antibody processes. It is composed of recombinant protein A coupled to the cross-linked 4% agarose base matrix.The highly crosslinked agarose resin enables high flow rates and high productivity.
Protein A resin characteristics
The recombinant protein is produced in E. coli and engineered with an oriented coupling to enhance binding capacity. Epoxy-based coupling ensures low ligand leakage. Specificity of the recombinant protein for the Fc region of IgG is similar to the native protein, giving a high degree of selectivity and yield.
Streamlined scale up
Scale-up with this resin is straightforward and is further aided by a range of prepacked process development tools, making purification scale-up straightforward.
특징 및 장점
- For monoclonal and polyclonal antibody purification at both laboratory and process scale.
- Well established among Protein A resins as excellent for monoclonal antibody (mAb) processes.
- Free from animal-derived components.
- Hydrophilic base matrix ensures low levels of non-specific binding and low levels of host cell-derived impurities in the elution pool.
- Fulfills industrial demands for security of supply, robust performance, and regulatory support.
저장 및 안정성
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
분석 메모
To view the Certificate of Analysis for this product, please visit www.cytiva.com.
법적 정보
Sepharose is a trademark of Cytiva
신호어
Warning
유해 및 위험 성명서
Storage Class Code
3 - Flammable liquids
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프로토콜
This page covers detailed information on cleaning procedures and recommended flow for column cleaning.
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