추천 제품
포장
pkg of 10 ea
제조업체/상표
Cytiva 11-0033-99
베드 용적
1 mL
컬럼 I.D.
15 mm
기질
6% cross-linked agarose
입자 크기
45-165 μm
평균 직경
90 μm
cleaning
2-14(Ni2+-stripped medium)
working range
3-12(Ni2+-stripped medium.)
용량
~40 mg binding capacity (histidine-tagged protein/column)(Protein binding capacity is protein-to-protein dependent.)
~40 mg binding capacity(histidine-tagged protein)
적합성
suitable for bioprocess medium
관련 카테고리
일반 설명
The ready to use column is prepacked with Ni Sepharose™ 6 Fast Flow, which has a high protein binding capacity, low nickel-ion leakage, and is compatible with denaturing and reducing agents, as well a wide range of additives.
His GraviTrap™ columns are delivered in a package that can be converted into a column stand to simplify purification. LabMate PD-10 Buffer Reservoir can be connected to the columns for convenient handling of sample volumes above 10 mL.
특징 및 장점
- For fast and simple purification of histidine-tagged* proteins.
- Convenient, prepacked gravity-flow column.
- Allows direct purification of both clarified and unclarified cell lysates.
- High binding capacity; approximately 40 mg of pure protein per column.
- Compatible with a wide range of reducing agents, detergents, denaturants and other additives.
저장 및 안정성
분석 메모
법적 정보
관련 제품
신호어
Warning
유해 및 위험 성명서
Storage Class Code
3 - Flammable liquids
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
문서
This page shows the characteristics of Ni Sepharose, Ni Sepharose excel, TALON Superflow, and uncharged IMAC Sepharose products from Cytiva.
This page describes manual and automated purification of histidine-tagged recombinant proteins using Cytiva products
프로토콜
This page shows how to purify histidine-tagged recombinant proteins by gravity flow using His GraviTrap and His GraviTrap Kit from Cytiva.
How to isolate proteins from inclusion bodies using Cytiva products.
This page shows how to perform sample desalting, buffer exchange and concentration for affinity chromatography of tagged proteins.
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