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GE11-0033-99

His GraviTrap

Cytiva 11-0033-99

동의어(들):

His GraviTrap Column

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About This Item

UNSPSC 코드:
12161501
NACRES:
NA.32

포장

pkg of 10 ea

제조업체/상표

Cytiva 11-0033-99

베드 용적

1 mL

컬럼 I.D.

15 mm

기질

6% cross-linked agarose

입자 크기

45-165 μm

평균 직경

90 μm

cleaning

2-14(Ni2+-stripped medium)

working range

3-12(Ni2+-stripped medium.)

용량

~40 mg binding capacity (histidine-tagged protein/column)(Protein binding capacity is protein-to-protein dependent.)
~40 mg binding capacity(histidine-tagged protein)

적합성

suitable for bioprocess medium

관련 카테고리

일반 설명

His GraviTrap is a prepacked, single-use column for purification of histidine-tagged proteins by immobilized metal affinity chromatography (IMAC). Column gives fast and simple gravity-flow purifications without any need for a purification.

The ready to use column is prepacked with Ni Sepharose 6 Fast Flow, which has a high protein binding capacity, low nickel-ion leakage, and is compatible with denaturing and reducing agents, as well a wide range of additives.

His GraviTrap columns are delivered in a package that can be converted into a column stand to simplify purification. LabMate PD-10 Buffer Reservoir can be connected to the columns for convenient handling of sample volumes above 10 mL.

특징 및 장점

  • For fast and simple purification of histidine-tagged* proteins.
  • Convenient, prepacked gravity-flow column.
  • Allows direct purification of both clarified and unclarified cell lysates.
  • High binding capacity; approximately 40 mg of pure protein per column.
  • Compatible with a wide range of reducing agents, detergents, denaturants and other additives.

저장 및 안정성

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)

분석 메모

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

법적 정보

GraviTrap is a trademark of Cytiva
Sepharose is a trademark of Cytiva

관련 제품

제품 번호
설명
가격

픽토그램

Environment

신호어

Warning

유해 및 위험 성명서

Storage Class Code

3 - Flammable liquids


시험 성적서(COA)

제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.

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문서 라이브러리 방문

문서

This page shows the characteristics of Ni Sepharose, Ni Sepharose excel, TALON Superflow, and uncharged IMAC Sepharose products from Cytiva.

This page describes manual and automated purification of histidine-tagged recombinant proteins using Cytiva products

프로토콜

This page shows how to purify histidine-tagged recombinant proteins by gravity flow using His GraviTrap and His GraviTrap Kit from Cytiva.

How to isolate proteins from inclusion bodies using Cytiva products.

This page shows how to perform sample desalting, buffer exchange and concentration for affinity chromatography of tagged proteins.

자사의 과학자팀은 생명 과학, 재료 과학, 화학 합성, 크로마토그래피, 분석 및 기타 많은 영역을 포함한 모든 과학 분야에 경험이 있습니다..

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