It is recommended to choose a molecular weight cut-off that is at least two times smaller than the molecular weight of the target molecules.
UFC5010
Amicon® Ultra Centrifugal Filter, 10 kDa MWCO
sample volume 0.5 mL, regenerated cellulose membrane, MWCO 10 kDa
동의어(들):
Centrifugal concentrator, 10 kDa MWCO, 0.5 mL sample volume
크기 선택
크기 선택
About This Item
추천 제품
material
polypropylene cap
polypropylene tube
regenerated cellulose membrane
styrene-butadiene housing
Quality Level
무균
non-sterile
제품 라인
Amicon®
제조업체/상표
Amicon® Ultra
파라미터
0.5 mL sample volume
기술
protein extraction: suitable
protein purification: suitable
길이
4.99 cm (1.96 in.)
직경
10.8 mm
여과 면적
1 cm2
용적
0.5 mL
공극 크기
10 kDa MWCO
배송 상태
ambient
일반 설명
The vertical design and available membrane surface area provide fast sample processing, high sample recovery (typically greater than 90% of dilute starting solution), and the capability for 30-fold concentration. Solute polarization and subsequent fouling of the membrane are minimized by the vertical design, and a physical deadstop in the filter device prevents spinning to dryness and potential sample loss. Efficient recovery of the concentrated sample (retained species) is achieved by a convenient reverse spin step after collecting the filtrate.
Amicon® Ultra-0.5 devices are supplied non-sterile and are for single use only. With their vertical membrane design and reverse spin, Amicon® Ultra 0.5 mL filters deliver great performance and lower spin times.
애플리케이션
- Ultrafiltration with 10,000 Dalton molecular weight cutoff Ultracel® membrane
- Concentration of biological samples containing antigens, antibodies, enzymes, nucleic acids, or microorganisms
- Purification of macromolecular components found in tissue culture extracts or cell lysates, primer removal, linkers, or macromolecular labels from a reaction mix, and protein removal prior to HPLC
- Desalting, buffer exchange, and protein dialysis
특징 및 장점
- Ultracel® regenerated cellulose membrane with 10 kDa NMWCO delivers retentate recovery of greater than 90%
- Concentrates 500 μL down to 15 μL
- Concentration factor 25x to 30x
- High sample recovery (greater than 90% of dilute starting solution)
- Fast processing time: 10 to 30 minutes
- Reverse spin capability provides consistent recoveries
물리적 형태
법적 정보
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시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
이미 열람한 고객
문서
Commercially available protein labeling kits provide researchers with the flexibility to customize their own immunoassay detection panels.
Protein purification is simpler with Amicon Pro. The device delivers consistent sample preparation for more reliable recovery, uncompromised purity and easier data generation.
PNGase Fast denaturing buffer and enzyme provide results similar to a conventional 20-hour protocol, reducing workflow time to about 1 hour.
나노입자 정제 및 생산에서 여과 방법 및 멤브레인 필터 선택.
프로토콜
Best practices for standardizing and reducing the Extracellular Vesicle Preparation workflow, and eliminating contamination of EV preparations with dead-cell-derived vesicles.
If an antibody must be purified from a small sample volume (0.5-10 mL), then the Amicon® Pro Purification System is recommended.
Detergents are frequently used to solubilize proteins and nucleic acids during purification, but the presence of detergents may interfere with downstream analyses.
관련 콘텐츠
Concentrate protein, don’t lose it. Recover protein reliably when you concentrate with Amicon® Ultra filters.
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What is the tolerance for molecular weight with these spin columns? Could you reasonably purify a protein of 15 kDa using these columns, or are the tolerances too high and would result in loss of protein into the filtrate?
1 답변-
도움이 되었습니까?
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Is this filter suitable for purifying MHC-bound peptides as filtrate/eluate while trapping proteins and antibodies as retentate following Immunoprecipitation?
1 답변-
This product is not suitable for separating protein/peptides of different sizes.
도움이 되었습니까?
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If a centrifuge is unable to achieve a spinning speed of 14000 X g as recommended in the protocol, is it possible to compensate by spinning the samples for a longer duration?
1 답변-
Yes, it is possible to compensate for a lower centrifuge spinning speed by increasing the duration. This can be achieved by calculating the K factor, which determines the adjustment needed in time.
도움이 되었습니까?
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se puede utilizar los UFC501024 para concentrar una proteina glicosilada?
1 답변-
Yes, Amicon devices like UFC501024 can be used to concentrate any protein greater than about 20 kDa.
도움이 되었습니까?
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Dear Sir/Mam, can you let me know how many tubes is 8 pkg? Is it 8 tubes? Best regards, Alejandra
1 답변-
It is 8 tubes per pack.
도움이 되었습니까?
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What is the maximum RPM that this can be spun to ensure the membrane does not perforate?
1 답변-
The recommended G force for the Amicon® Ultra-0.5 filter is 14,000 g & 1,000 g reverse spin when using a Fixed-Angle (35 º) Rotor.
Please refer to the brochure for more information: details: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/260/181/br5002en-protein-prep-workflow-ms.pdf
도움이 되었습니까?
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