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Merck
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주요 문서

UFC5010

Millipore

Amicon® Ultra Centrifugal Filter, 10 kDa MWCO

Amicon® Ultra Centrifugal Filter, 10 kDa MWCO
1명의 제품 평가자(총 2명)가 샘플 제품을 받았거나 프로모션에 참여했습니다

sample volume 0.5 mL, regenerated cellulose membrane, MWCO 10 kDa

동의어(들):

Centrifugal concentrator, 10 kDa MWCO, 0.5 mL sample volume

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크기 선택

8 PKG
₩86,275
24 PKG
₩214,774
96 PKG
₩763,077
500 PKG
₩3,142,237

₩86,275


구입 가능 여부는 고객센터에 문의하십시오.

벌크 견적 요청

크기 선택

보기 변경
8 PKG
₩86,275
24 PKG
₩214,774
96 PKG
₩763,077
500 PKG
₩3,142,237

About This Item

UNSPSC 코드:
41104916
eCl@ss:
36100101
NACRES:
NB.24
material:
polypropylene cap
polypropylene tube
regenerated cellulose membrane
styrene-butadiene housing
공극 크기:
10 kDa MWCO
길이:
4.99 cm (1.96 in.)
직경:
10.8 mm
여과 면적:
1 cm2
용적:
0.5 mL
무균:
non-sterile

₩86,275


구입 가능 여부는 고객센터에 문의하십시오.

벌크 견적 요청

material

polypropylene cap
polypropylene tube
regenerated cellulose membrane
styrene-butadiene housing

Quality Level

무균

non-sterile

제품 라인

Amicon®

제조업체/상표

Amicon® Ultra

파라미터

0.5 mL sample volume

기술

protein extraction: suitable
protein purification: suitable

길이

4.99 cm (1.96 in.)

직경

10.8 mm

여과 면적

1 cm2

용적

0.5 mL

공극 크기

10 kDa MWCO

배송 상태

ambient

일반 설명

Amicon® Ultra-0.5 centrifugal filter devices provide fast ultrafiltration, with the capability for high concentration factors and easy concentrate recovery from dilute and complex sample matrices. For example, Cytochrome c (12.4 kDa) at 0.25 mg/ml initial concentration is typically retained at >95% .

The vertical design and available membrane surface area provide fast sample processing, high sample recovery (typically greater than 90% of dilute starting solution), and the capability for 30-fold concentration. Solute polarization and subsequent fouling of the membrane are minimized by the vertical design, and a physical deadstop in the filter device prevents spinning to dryness and potential sample loss. Efficient recovery of the concentrated sample (retained species) is achieved by a convenient reverse spin step after collecting the filtrate.

Amicon® Ultra-0.5 devices are supplied non-sterile and are for single use only. With their vertical membrane design and reverse spin, Amicon® Ultra 0.5 mL filters deliver great performance and lower spin times.

애플리케이션

  • Ultrafiltration with 10,000 Dalton molecular weight cutoff Ultracel® membrane
  • Concentration of biological samples containing antigens, antibodies, enzymes, nucleic acids, or microorganisms
  • Purification of macromolecular components found in tissue culture extracts or cell lysates, primer removal, linkers, or macromolecular labels from a reaction mix, and protein removal prior to HPLC
  • Desalting, buffer exchange, and protein dialysis

특징 및 장점

  • Ultracel® regenerated cellulose membrane with 10 kDa NMWCO delivers retentate recovery of greater than 90%
  • Concentrates 500 μL down to 15 μL
  • Concentration factor 25x to 30x
  • High sample recovery (greater than 90% of dilute starting solution)
  • Fast processing time: 10 to 30 minutes
  • Reverse spin capability provides consistent recoveries

물리적 형태

Color Code: Clear

법적 정보

Amicon is a registered trademark of Merck KGaA, Darmstadt, Germany
ULTRACEL is a registered trademark of Merck KGaA, Darmstadt, Germany

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시험 성적서(COA)

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이 제품을 이미 가지고 계십니까?

문서 라이브러리에서 최근에 구매한 제품에 대한 문서를 찾아보세요.

문서 라이브러리 방문

문서

Commercially available protein labeling kits provide researchers with the flexibility to customize their own immunoassay detection panels.

Protein purification is simpler with Amicon Pro. The device delivers consistent sample preparation for more reliable recovery, uncompromised purity and easier data generation.

PNGase Fast denaturing buffer and enzyme provide results similar to a conventional 20-hour protocol, reducing workflow time to about 1 hour.

나노입자 정제 및 생산에서 여과 방법 및 멤브레인 필터 선택.

모두 보기

프로토콜

Best practices for standardizing and reducing the Extracellular Vesicle Preparation workflow, and eliminating contamination of EV preparations with dead-cell-derived vesicles.

If an antibody must be purified from a small sample volume (0.5-10 mL), then the Amicon® Pro Purification System is recommended.

Detergents are frequently used to solubilize proteins and nucleic acids during purification, but the presence of detergents may interfere with downstream analyses.

관련 콘텐츠

Concentrate protein, don’t lose it. Recover protein reliably when you concentrate with Amicon® Ultra filters.

질문

1–6 / 6 질문  
  1. What is the tolerance for molecular weight with these spin columns? Could you reasonably purify a protein of 15 kDa using these columns, or are the tolerances too high and would result in loss of protein into the filtrate?

    1 답변
    1. It is recommended to choose a molecular weight cut-off that is at least two times smaller than the molecular weight of the target molecules.

      도움이 되었습니까?

  2. Is this filter suitable for purifying MHC-bound peptides as filtrate/eluate while trapping proteins and antibodies as retentate following Immunoprecipitation?

    1 답변
    1. This product is not suitable for separating protein/peptides of different sizes.

      도움이 되었습니까?

  3. If a centrifuge is unable to achieve a spinning speed of 14000 X g as recommended in the protocol, is it possible to compensate by spinning the samples for a longer duration?

    1 답변
    1. Yes, it is possible to compensate for a lower centrifuge spinning speed by increasing the duration. This can be achieved by calculating the K factor, which determines the adjustment needed in time.

      도움이 되었습니까?

  4. se puede utilizar los UFC501024 para concentrar una proteina glicosilada?

    1 답변
    1. Yes, Amicon devices like UFC501024 can be used to concentrate any protein greater than about 20 kDa.

      도움이 되었습니까?

  5. Dear Sir/Mam, can you let me know how many tubes is 8 pkg? Is it 8 tubes? Best regards, Alejandra

    1 답변
    1. It is 8 tubes per pack.

      도움이 되었습니까?

  6. What is the maximum RPM that this can be spun to ensure the membrane does not perforate?

    1 답변
    1. The recommended G force for the Amicon® Ultra-0.5 filter is 14,000 g & 1,000 g reverse spin when using a Fixed-Angle (35 º) Rotor.

      Please refer to the brochure for more information: details: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/260/181/br5002en-protein-prep-workflow-ms.pdf

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