G5507

Galactosyltransferase from bovine milk

lyophilized powder

• 동의어(들): Lactose Synthase, UDP-galactose:D-glucose 4-β-galactosyltransferase
• CAS Number: 9031-68-9
• 효소 위원회 번호: 2.4.1.22 (BRENDA, IUBMB)
• MDL number: MFCD00131122
• UNSPSC 코드: 12352204
• NACRES: NA.54

속성

• 양식: lyophilized powder
• Quality Level: 200
• 특이 활성도: 3.0-15.0 units/mg protein (in the presence of adequate α-lactalbumin, without added α-lactalbumin the activity is zero)
• 구성: Protein, 35-65% modified Warburg-Christian
• 불순물: ≤10 % protein α-lactalbumin
• 저장 온도: −20°C

설명

일반 설명

Research area: Cell signalling. Galactosyltransferase(GalT) is found in both prokaryotes and eukaryotes.[1]Galactosyltransferases belong to the galactosyltransferase family and are anchored to the membrane of Golgi through their N-terminal region.[2]

애플리케이션

Galactosyltransferase from bovine milk has been used:

• in the generation of hyper-galactosylated variant of IgG1[3]
• in β -1,4-galactosyltransferase assay rice root cell extracts[4]
• in the modification of LacNAcQ11 and LacDiNAcQ11 nanofibers[5]

Galactosyltransferase is the catalytic component of the lactose synthetase system; it synthesizes lactose slowly in the absence of the regulatory protein α-lactalbumin. Galactosyltransferase will also transfer galactose from UDP-galactose to N-acetylglucosamine. This preparation is useful in the determination of α-lactalbumin, UDP-galactose, and N-acetylglucosamine.

생화학적/생리학적 작용

Galactosyltransferase catalyzes the transfer of galactosyl molecules in the synthesis of oligosaccharides[6].

Galactosyltransferase from bovine milk requires Mn²⁺ ions for its catalytic functionality.[7] Alteration in the galactosyltransferase levels have been implicated in various disease states.[2]Galactosyltransferase(GalT) functions either as a soluble enzyme or as membrane-bound moieties.[1]

결합

This is probably the same enzyme as N-acetyllactosamine synthetase, EC 2.4.1.38 and EC 2.4.1.90.

단위 정의

One unit will transfer 1.0 μmole of galactose from UDP-galactose to D-glucose per min at pH 8.4 at 30 °C in the presence of 0.2 mg α-lactalbumin per mL of reaction mixture. (without added α-lactalbumin the activity is zero)

물리적 형태

Lyophilized powder containing Tris, EDTA, and (NH₄)₂SO₄

안전성 정보

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: Eyeshields, Gloves, type N95 (US)